PEROXIDASE IN SALIVA AND PREVENTION OF ORAL DISEASE

乳过氧化物酶(LP)、过氧化氢(H_2O_2)和硫氰酸盐离子(SCN-) 在人类唾液中形成抑菌系统。口服“乳酸片” 细菌“释放过氧化氢作为碳水化合物代谢的副产品。 催化过氧化氢氧化SCN-生成次硫氰酸根离子 (OSCN-)，与次硫氰酸处于酸碱平衡状态 (HOSCN)。HOSCN氧化细菌酶的基本巯基 和运输系统，导致新陈代谢和生长受到抑制。 某些细菌，如变形链球菌，对抑制的抵抗力有限， 由于它们能够迅速将某些二硫化合物还原为 形成了巯基。这些低摩尔的。羟基化合物保护重量 必需蛋白质通过与抑制剂反应和还原而生成 它回到了SCN-。这些研究将评估可能增加LP的药物 唾液中的抗菌作用：(1)通过增加唾液中的过氧化氢供应 为了克服对抑制的有限抵抗力，(2)通过增加 抑制物的稳定性和/或反应性，以及(3)通过干扰 负责抵抗的活动。更有前途的特工就会 与变形链球菌、其他口腔链球菌和链球菌一起研究 不要将口腔定为目标微生物。待测试的药物 包括氨基糖作为导致过氧化氢增加的底物 生产，过氧化氢和烷基过氧化氢，葡萄糖氧化酶，白花丹，碘化物， 磺胺化合物、氰化物和抑制剂或竞争底物 参与抵抗的活动。这些研究将提供 有关LP系统与细菌相互作用的信息 可以在这种抗菌系统的存在下生存和生长。这个 研究还应为评估 LP系统在口腔疾病天然抵抗力中的应用(1)开发方法 目的：检测可用于临床的LP抗菌活性。 环境，以及(2)通过确定可以提高疗效的制剂 或对口腔病原体的抗菌作用的选择性。这样的代理人 可以用来比较LP系统在正常情况下的效果 在接受代理的总体中产生影响的总体。

Lactoperoxidase (LP), hydrogen peroxide (H2O2) and thiocyanate ion (SCN-) form a bacteriostatic system in human saliva. The oral "lactic-acid bacteria" release H2O2 as a by-product of carbohydrate metabolism. LP catalyzes the oxidation of SCN- by H2O2 to yield hypothiocyanite ion (OSCN-), which is in acid-base equilibrium with hypothiocyanous acid (HOSCN). HOSCN oxidizes essential sulfhydryl groups of bacterial enzymes and transport systems, resulting in inhibition of metabolism and growth. Certain bacteria such as S. mutans have a limited resistance to inhibition, due to their ability to rapidly reduce certain disulfide compounds to the sulfhydryl forms. These low-mol. wt. sulfhydryl compounds protect essential protein sulfhydryls by reacting with the inhibitor and reducing it back to SCN-. These studies will evaluate agents that may increase LP antimicrobial action in saliva: (1) by increasing the peroxide supply so as to overcome the limited resistance to inhibition, (2) by increasing the stability and/or reactivity of the inhibitor, and (3) by interfering with the activities responsible for resistance. The more promising agents will be studied with S. mutans, other oral streptococci, and streptococci that do not colonize the mouth as target microorganisms. Agents to be tested include amino sugars as substrates that result in increased H2O2 production, H2O2 and alkyl peroxides, glucose oxidase, plumbagan, iodide, sulfonamide compounds, cyanide, and inhibitors or competitive substrates of the activities involved in resistance. These studies will provide information about the interaction of the LP system with the bacteria that can survive and grow in the presence of this antimicrobial system. The studies should also provide a basis for evaluating the significance of the LP system in natural resistance to oral disease, (1) by developing methods to measure LP antimicrobial activity that could be used in the clinical environment, and (2) by identifying agents that can increase the efficacy or selectivity of antimicrobial action against oral pathogens. Such agents could then be used to compare the effect of the LP system in the normal population with the effect in a population receiving the agent.