ANTIBODY TO GLUCOSYLTRANSFERASE IN ADULTS AND CHILDREN

对口腔抗原的唾液免疫应答可以调节口腔粘膜上皮细胞的数量和分布。 一种寄生在口腔中的微生物。 这些反应可能 通过暴露于肠道中的抗原或通过暴露于淋巴细胞引起的， 口腔中的组织，例如小唾液腺。 一个 了解婴儿免疫能力的发展， 幼儿，以及儿童第一次 对口腔链球菌如S. salivrius，knowleable时尚. 葡萄糖基转移酶是这些链球菌的胞外产物， 合成葡聚糖，其中一些涉及 S.变异人 为此，本说明建议： 以测量伊加抗体水平的纵向发展， 葡萄糖基转移酶抗原。salivrius，S. sanguis和S.变形 在婴儿和五岁以下儿童的整个唾液中。 的 这些口腔链球菌的存在也将通过选择性的 牙菌斑或口腔粘膜的平板培养，以识别最初的外观 这些链球菌。 这些微生物的鉴定将是 与相应抗原的抗体水平相关。 的 唾液抗体的同种型分布，特别是在来自 六个月以下的孩子，会因为高压而窒息。 液相色谱法和酶联免疫吸附试验（ELISA）。 的 2、4和6月龄口服脊髓灰质炎婴儿的抗原激发 疫苗和肠外百白破疫苗是一个有用的模型，探索 口腔黏膜免疫耐受形成过程中的免疫应答 腔 因此，对外观和变化进行纵向研究， 1、2和3型脊髓灰质炎病毒和破伤风唾液抗体水平 将在5岁以下儿童中进行类毒素试验。 最后 在口腔中引发局部免疫应答的能力将是 通过将破伤风类毒素应用于唇粘膜进行研究。 将通过ELISA法测定小阴唇腺分泌物的反应。 这些数据应揭示局部抗原刺激的潜力， 口腔和使用这种途径的未来免疫方案。

The salivary immune response to oral antigens may modulate the number and type of microorganisms which colonize the oral cavity. These responses may be elicited by exposure to antigen in the gut or by exposure of lymphtic tissue in the oral cavity, for example, in minor salivary glands. An understanding of the development of immunocompetence in the infant and young child, and an identifiction of the ages at which children first respond to oral streptococci, such as S. salivrius, knowlegeable fashion. Glucosyltransferases are extracellular products of these streptococci which synethesize glucans, some of which have been implicated in the cariogenicity of S. mutans. To this end, the present investigtion proposes to measure the longitudinal development of IgA antibody levels to glucosyltransferase antigens from S. salivrius, S. sanguis, and S. mutans in whole salivas of infants and children up to five years of age. The presence of these oral streptococci will also be ascertained by selective plating of plaque or oral mucosa in order to identify the irst appearance of these streptococci. The identification of these microorganisms will be correlated with antibody levels to the repective antigens. The distribution of isotypes of salivary antibody, especially in salivas from children younger than six months, will be investigted by high pressure liquid chromatography and enzyme-linked immunosorben assay (ELISA). The antigenic challenge of infants at 2,4 and 6 months of age with oral polio vaccine and parenteral DPT vaccine represents a useful model for exploring immune responses during the development of immuocompetance in the oral cavity. Thus, a longitudinal study of the appearance and changes in salivary antibody levels to poliovirus typs 1, 2 and 3 and to tetanus toxoid will be conducted in children up to five yers of age. Finally, the ability to elicit a local immune response in the oral cavity will be investigated by application of tetanus toxoid to the labial mucosae. Responses will be measured by ELISA in labial minor gland secretions. These data should reveal the potential for local antigenic stimulation in the oral cavity and the use of this route for future immunization protocols.