ROLE OF INNERVATION IN ELECTRORECEPTOR REGENERATION

弱电鱼的结节状电感受器与毛发有关 侧线和内耳的细胞。 就像一些毛细胞一样， 通过基底膜中的电子过滤器进行调谐。 的数量 在鱼的一生中，每个器官中的感觉细胞都会增加， 感觉细胞由支持细胞产生。 此外，当皮肤 新的皮肤在伤口和结节状受体器官中再生 我们相信，再生是从完整器官的支持细胞中产生的。 周围的皮肤迁移到再生皮肤。 期间 再生结节器官开始作为一层支持细胞周围 这些支持细胞也会产生新的感觉细胞。 这些受体显示出对它们在该受体中的神经支配的依赖性 器官被破坏，并失去感觉受体细胞2-3周后， 去神经 受体器官也不会出现在失神经支配的皮肤。 我们希望在本提案中回答以下问题。 1)并 失神经后受体细胞调谐的变化？ 2)什么形态学 当受体退化时，它们会发生变化，如果有的话， 发生在支持细胞中？ 3)神经支配在 刺激支持细胞进行有丝分裂，并在细胞中添加新的感觉细胞。 完整的器官 4)神经支配在早期脑缺血中起什么作用 结节性感觉器官的再生，如果 新的皮肤周围是失去神经支配的旧器官 5)是新 再生器官对去神经支配特别敏感？ 6)利率如何 失神经支配后再生器官支持细胞有丝分裂的变化？ 这项研究的结果将增加我们对 感觉细胞再生的过程和假定的营养影响 感觉细胞上的传入神经。

The tuberous electroreceptors of weakly electric fish are related to hair cells of the lateral line and inner ear. Like some hair cells, they are tuned by an electrical filter in their basal membranes. The number of sensory cells in each organ increases throughout the fish's life, the new sensory cells being produced by support cells. Furthermore, after skin has been removed new skin regrows in the wound and tuberous receptor organs regenerate, we believe, from the support cells of intact organs in the surrounding skin that migrate into the regenerating skin. During regeneration the tuberous organ begins as a layer of support cells around a lumen, and these support cells also give rise to the new sensory cells. These receptors show a dependence on their innervation in that receptor organs become disrupted and lose sensory receptor cells 2-3 weeks after denervation. Receptor organs also do not appear in denervated skin. We wish to answer the following questions in this proposal. 1) Does receptor cell tuning change after denervation? 2) What morphological changes occur in receptors as they degenerate and what changes, if any, occur in the support cells? 3) What role does innervation play in stimulating support cells to become mitotic and add new sensory cells in intact organs? 4) What role does innervation play in the early regeneration of tuberous sensory organs and, do organs regenerate if the new skin is surrounded by old organs that are denervated? 5) Are newly regenerated organs especially sensitive to denervation? 6) How do rates of mitosis change in support cells of regenerating organs after denervation? The results from this study will increase our fundamental understanding of the process of sensory cell regeneration and the putative trophic influence of afferent nerves upon their sensory cells.