CELL SURFACE INTERACTIONS IN ORAL PLAQUE BACTERIA

玉米芯是由有序的 丝状微生物和链球菌的共聚集。在… 粘着的链球菌覆盖在表面上 花丝使复合体看起来像一穗玉米穗。这些 在发育或成熟过程中观察到了玉米芯结构 人类的牙菌斑，有人认为玉米芯 队形是连接以上和 牙龈下菌斑。我们早期的工作表明梭杆菌 核杆菌，一种被认为是厌氧革兰氏阴性细菌 患有某些形式的牙周病，有能力 在体外，与血链球菌菌株形成玉米芯。 一个目标是描述以前确定的 核盘藻ATCC 10953玉米芯受体的构建 用于检测和分析的免疫学和遗传学探针 这种受体，并开发旨在抑制或阻断 玉米芯的体外反应。一种具有胰酶活性的多肽 建议的玉米芯受体将在制备过程中分离出来 用单特异性免疫亲和层析法测定鳞片 多克隆抗体。该多肽将用 经典的生化程序和氨基末端部分 该分子将被测序。由此得出的信息 序列将应用于DNA杂交的生产 将用于检测克隆的 重组质粒中的结构基因，以及基因的测定 玉米芯受体在口腔临床中的分布 核梭杆菌的分离株。抗受体抗体 和精选的多肽，来自于蛋白质水解性消化 受体蛋白将以竞争和直接两种方式进行评估 结合研究，关于防止玉米芯形成的能力 体外培养。免疫学和遗传学的发展和应用 探测器应该会极大地增强我们定义复合体的能力 发生在口腔中的属间共聚集机制 空洞。此外，结果应该导致生产高炉。 可用于防止斑块成熟的试剂 并减少一种可能的炎症机制的发生率 与牙周炎和牙周炎的进展有关。

Corncobs are distinct morphological units formed by the ordered coaggregation of a filamentous microorganism and streptococci. At saturating numbers the adherent streptococci coat the surface of the filament so that the complex resembles an ear of corn. These corncob structures have been observed in developing or maturing human dental plaque and it has been suggested that corncob formation represents a connecting link between supra- and subgingival plaque. Our earlier work has shown that Fusobacterium nucleatum,an anaerobic gram-negative bacterium thought to be involved in some forms of periodontal disease, has the ability to form corncobs, in vitro, with strains of Streptococcus sanguis. One objectives are to characterize the previously identified corncob-receptor of F. nucleatum ATCC 10953, construct immunological and genetic probes for the detection and analysis of this receptor, and develop strategies designed to inhibit or block the corncob reaction in vitro. An active tryptic polypeptide of the proposed corncob receptor will be isolated on a preparative scale by immunoaffinity chromatography using monospecific polyclonal antibodies. The polypeptide will be characterized using classical biochemical procedures and the amino terminal portion of the molecule will be sequenced. Information derived from this sequence will be applied to the production of DNA hybridization probes which will be used for both the detection of the cloned structural gene, in recombinant plasmids, and the determination of the distribution of this specific corncob receptor in oral clinical isolates of Fusobacterium nucleatum. The anti-receptor antibodies and selected peptides, derived from the proteolytic digestion of the receptor protein will be evaluated, in competition and direct binding studies, for the ability to prevent corncob formation in vitro. Development and application of immunological and genetic probes should greatly enhance our ability to define the complex intergeneric coaggregation mechanisms that occur in the oral cavity. Furthermore, the results should lead to the production bf reagents which could be used to prevent the maturation of plaque and reduce the incidence of one possible inflammatory mechanism associated with the progression of gingivitis and periodontitis.