ANTIBODY TO GLUCOSYLTRANSFERASE IN ADULTS AND CHILDREN

对唾液免疫反应重要的因素可以有效地改变 病原微生物区系的定植和/或毒力 发展，亚类限制，抗原给药途径，抗原 演示文稿和回忆。婴儿体内仍处于成熟阶段的分泌物 免疫系统面临日益复杂的传染病挑战 由潜在的致病口腔菌群引起。在以后的生活中，可用的路径 对于保护性的分泌性免疫反应是多种多样的，包括 在微环境中表达分泌性免疫的独特系统 整个口腔，即小唾液腺网络。这 该提案将探讨在婴幼儿中， 分泌型免疫系统通过与原住民的相互作用 微生物群：1)唾液抗体发展的比较 口腔感染的酶联免疫吸附试验检测口腔链球菌抗原 链球菌；2)唾液进行性复杂性的测量 婴儿自身口腔菌群表位的抗体反应 Blotting；3)初始唾液个体发育测量的鉴定 百日咳杆菌抗原和记忆剂注射后的抗体反应 对注射(破伤风类毒素)和口服(脊髓灰质炎病毒)的反应 疫苗，在ELISAb中。还将探索成熟的潜力 唾液免疫在小(唇)涎腺唾液中的表达 5)用EL ISA-Ig法检测儿童LS中的Ig G和Ig A浓度， 青少年、年轻人和老年人；6)衡量 LsIgA和Ig G同型和亚类抗体，用a)Western印迹 LS、腮腺和血清对一组口服抗原抗体的分析， B)抗体谱的等电聚焦/Western印迹分析 和7)探索刺激GLS的可能性 免疫球蛋白和免疫球蛋白抗体及分泌抗体的外周血单核细胞 局部或系统免疫破伤风类毒素，以及8) 联合全身免疫增强LS抗体应答的可能性 局部应用抗原的免疫。这些研究应该a) 提供关于唾液免疫个体发生的新的基本信息， B)确定在初始阶段具有免疫原性的毒力抗原 殖民期，因此可以被合并到牙科 龋齿疫苗，c)有助于阐明小唾液腺的作用 口服免疫中的网络，以及d)确定特定的 通过免疫增强LS免疫力。

Factors important to salivary immune responses which can effectively modify colonization and/or virulence of pathogenic microbiota include ontogenic development, subclass restriction, route of antigen administration, antigen presentation, and anamnesis. In the infant a still-maturing secretory immune system is confronted with increasingly complex infectious challenges by potentially pathogenic oral flora. Later in life the pathways available for protective secretory immune responses are more varied and include a unique system for expression of secretory immunity in microenvironments throughout the oral cavity, namely, the minor salivary gland network. This proposal will explore, in infants and young children, the development of the secretory immune system through its interaction with indigenous microbiota by: 1) comparison of the development of salivary antibody to oral streptococcal antigens in ELISA-Ab with infection by oral streptococci; 2) measurement of the progressive complexity of salivary antibody responses to epitopes from an infant's own oral flora by Western blotting; 3) identification of the ontogeny measurement of initial salivary antibody response to injection of B.pertussis antigens and anamnestic responses to injected (tetanus toxoid) and orally administered (poliovirus) vaccines, in ELISA-Ab. Also explored will be the potential for mature salivary immune expression in minor (labial) salivary gland saliva (LS) by 5) identifying IgG and IgA concentration by ELISA -Ig in LS from children, adolescents, young and older adults; 6) measuring the distinctiveness of LS IgA and IgG isotypic and subclass antibody, using a) Western blot analysis of LS, parotid and serum antibody to a battery of oral antigens, and b) isoelectric focusing/Western blot analysis of antibody spectrotypes to individual antigens; and 7) exploring the potential for stimulation gLS IgA and IgG antibody and antibody-secreting peripheral blood monocytes by topical or systemic immunization with tetanus toxoid, and 8) the possibility for enhancing LS antibody responses by combining systemic immunization with local application of antigen. These studies should a) provide new basic information about the ontogeny of salivary immunity, b)identify virulence antigens which are immunogenic during the initial colonization period and which thus could be incorporated into a dental caries vaccine, c) help to clarify the role of the minor salivary gland network in oral immunity, and d) identify the potential for specific enhancement of LS immunity by immunization.