PERIODONTAL SHORT CHAIN FATTY ACIDS TOXIC TO PMNS

多形核白细胞（PMNs）是免疫系统的步兵。 细胞宿主防御系统 感染后PMNs 表现出一系列细胞功能的增加，包括： 粘附、趋化性、吞噬作用、脱粒作用， 杀菌 这些反应中的前四个涉及细胞 运动性，因此依赖于持续的，有序的，长期的 肌动蛋白细胞骨架的重组。 积累数据 表明受体-配体相互作用启动这些细胞 应答 此外，早期的事件之后， 受体-配体相互作用包括立即的，快速的， 肌动蛋白细胞骨架和细胞内 博士 在单独但相关的文献中，临床数据表明， 厌氧细菌被认为是导致牙周炎的原因， 感染释放短链脂肪酸。（SCFA）进入他们的 环境 这些SCFA已显示抑制PMN运动， 功能协调发展的 因此，我们有兴趣回答以下分子 问题： 1. SCFAs改变PMN肌动蛋白细胞骨架吗？ 2. SCFAs会改变PMN的内部pH值吗？ 和 3. SCFA改变PMN趋化肽受体吗？ 如果拟议工作的结果表明SCFA确实 对PMNs有有害影响，几个额外的问题 将引起极大的临床兴趣：临床试验机构中存在哪些SCFA 牙周炎患者吗 SCFA对其他人的影响是什么 牙周组织的细胞 最后，细菌细胞能被 减少了SCFA的产量？

Polymorphonuclear leukocytes (PMNs) are the infantry of the cellular host defense system. In response to infections PMNs exhibit an increase in a sequence of cell functions which include: adhesion, chemotaxis, phagocytosis, degranulation, and ultimately bacterial killing. The first four of these responses involve cell motility, and therefore depend on continued, orderly, long-term reorganizations of the actin cytoskeleton. Accumulating data indicate that receptor-ligand interactions initiate these cellular responses. Furthermore, the very early events following receptor-ligand interaction include an immediate, rapid, transient, alteration in the actin cytoskeleton and intracellular pH. In separate but related literature, clinical data indicate that anaerobic bacteria which are thought to cause periodonatal infections release short chain fatty acids. (SCFA) into their environment. These SCFA have been shown to inhibit PMN motile functions. We are therefore interested in answering the following molecular questions: 1. Do SCFAs alter the PMN actin cytoskeleton? 2. Do SCFAs alter the internal pH of the PMN? and 3. Do SCFAs alter PMN chemotactic peptide receptors? If the results of the proposed work indicate that the SCFAs do have a deleterious effect on PMNs, several additional questions will be of great clinical interest: What SCFAs are present in sites with active periodontitis? What is the effect of SCFA on other cells of the periodontium? and finally, Can bacterial cells be engineered which have reduced SCFA production?