茯砖茶多糖（FBTPS）具有调节宿主脂代谢的活性，前期研究表明FBTPS可能是通过调节肠道菌群中Bacteroides plebeius进而调节宿主脂代谢，然而FBTPS与B. plebeius互作机制尚不清楚。FBTPS-2-1是FBTPS主要活性组分，本项目拟利用单菌厌氧发酵模型结合代谢组学和转录组学研究FBTPS-2-1与B. plebeius互作关系，通过B. plebeius全基因组测序和转录组学预测并挖掘参与水解FBTPS-2-1的多糖利用位点（PUL），并利用大肠杆菌表达潜在碳水化合物活性酶（CAZymes）。通过酶学特性验证CAZymes代谢FBTPS-2-1的活性。本项目以关键PULs和CAZymes的挖掘为切入点系统研究FBTPS-2-1与B. plebeius互作机制，研究结果不仅可以阐明FBTPS调节脂代谢的可能机理，而且可以为茯砖茶的生产和高值化利用提供理论支撑。

Fuzhuan brick tea polysaccharides (FBTPS) could modulate the host lipid metabolism. The previous work showed the regulation of host lipid metabolism by FBTPS may be related to the modulation of Bacteroides plebeius in gut microbiota. However, the mechanism of interaction between FBTPS and B. plebeius is still unclear. FBTPS-2-1 is the main fraction in FBTPS. In this project, the interaction relationship between FBTPS-2-1 and B. plebeius is evaluated by model of anaerobic fermentation of single strain as well as metabolomics and transcriptomics. The potential polysaccharide Utilization Loci (PUL) for hydrolysis of FBTPS-2-1 was predicted and excavated by metagenome sequencing and transcriptomics of B. plebeius. The potential carbohydrate active enzymes (CAZymes) were expressed by Escherichia coli. The metabolism of FBTPS-2-1 by CAZymes was confirmed by evaluating enzymatic characterization of CAZymes. This project study the interaction of FBTPS-2-1 with B. plebeius based on the excavation of key PULs and CAZymes, which may not only clarify the possible mechanism of FBTPS in regulating lipid metabolism, but also provide theoretical support for the production and high-value utilization of Fuzhuan brick tea.