FIBRINOGEN BINDING AND VIRULENCE OF BACTEROIDES SPECIES

某些形式的牙周炎和牙周炎与 土著黑色素的比例增加(出现) 龈下微生物区系中类杆菌的种类，特别是 牙龈拟杆菌和中间类杆菌。它不是 知道哪些致病因素或哪些因素允许出现 这些生物体，或者这些生物体的出现 有助于牙周炎和牙周炎的发病。 许多牙龈假单胞菌和中间假单胞菌菌株结合并 体外黏附纤维蛋白原和纤维蛋白。约束和坚守 这些菌株的纤维蛋白原是由一种细菌蛋白(MR)介导的 200K)。有研究建议调查纤维蛋白原是否 结合蛋白(FBP)是体内必需的毒力因子 牙周炎病变发展过程中牙龈假单胞菌的出现 食蟹猴。致病机制的阐明 类杆菌属物种的出现对理解 它们在牙周疾病中的病因学作用。一种对 这些机制对于实现长期目标也是必要的。 这项研究的目的是开发阻止涌现的策略 从而阻止了这些生物的发展 牙周炎。提出了以下具体目标： 1.纯化人和人纤维蛋白原结合蛋白 食蟹类杆菌牙龈类杆菌和类杆菌 中间人。 2.鉴定和比较大鼠纤维蛋白原结合蛋白 猴和人牙龈类杆菌和 中间类杆菌。 3.确定牙龈类杆菌与纤维蛋白原结合 蛋白质是食蟹猴体内的毒力因子 大鼠牙周炎模型的建立： (1)测定对牙龈假单胞菌的主动免疫力 (A)防止出现牙龈假单胞菌或其他类杆菌 在发展中的牙周炎病变的微生物区系中的物种， 或 (B)防止牙周炎的发展和 (2)确定牙龈假单胞菌突变体是否缺乏纤维蛋白原结合 蛋白质(FBP-)可以植入并出现在 发展中的牙周炎病变。

Some forms of gingivitis and periodontitis are associated with proportional increases (emergence) of indigenous black-pigmented Bacteroides species in the subgingival microbiota, particularly Bacteroides gingivalis and Bacteroides intermedius. It is not known what virulence factor or factors permit emergence of these organisms, or whether emergence of these organisms contributes to the pathogenesis of gingivitis and periodontitis. A number of B. gingivalis and B. intermedius strains bind and adhere to fibrinogen and fibrin in vitro. Binding and adherence of these strains to fibrinogen are mediated by a bacterial protein (Mr 200k). Studies are proposed to investigate whether fibrinogen binding protein (FBP) is an in vivo virulence factor necessary for emergence of B. gingivalis in developing periodontitis lesions in the cynomolgus monkey. Elucidation of mechanisms of emergence of Bacteroides species is important in understanding their etiologic role in periodontal diseases. An understanding of these mechanisms is also necessary to achieve the long range goal of this research which is to develop strategies to block emergence of these organisms and thereby block the development of periodontitis. The following Specific Aims are proposed: 1. To purify fibrinogen binding proteins from human and cynomolgus strains of Bacteroides gingivalis and Bacteroides intermedius. 2. To characterize and compare fibrinogen binding proteins from monkey and human strains of Bacteroides gingivalis and Bacteroides intermedius. 3. To determine if Bacteroides gingivalis fibrinogen binding protein is an in vivo virulence factor in the cynomolgus monkey model of ligature-induced periodontitis by: (1) determining if active immunity to B. gingivalis FBP (a) prevents emergence of B. gingivalis or other Bacteroides species in the microbiota of developing periodontitis lesions, or (b) prevents development of periodontitis and (2) determining if B. gingivalis mutants lacking fibrinogen binding protein (FBP-) can implant and emerge in the microbiota of developing periodontitis lesions.