ION CHANNELS IN SALIVARY GLAND DUCT CELLS

这些研究的目的是确定离子 唾液腺导管功能的基础电流， 阐明调节这些电流的细胞内因素。 其方法是采用贴片电生理技术 夹紧，以补充和扩展使用 放射性示踪技术 推导出待检验的假设 从我们的初步研究中获得的结果来看， 唾液腺腺泡细胞的电生理学研究，以及 一般测试是否相同的离子电流和电流 存在于腺泡细胞中的调节机制也存在于 导管细胞 这些研究利用全细胞电流测量 来自未刺激和毒蕈碱刺激的HSG-PA组织 培养细胞，并对切除的细胞进行单通道电流测量。 这些细胞的膜补丁。 全细胞研究现状 允许分析和表征静息细胞膜的生物活性， 以及涉及的各种细胞内因子的作用 在细胞信号传导如G蛋白、IP3、Ca离子浓度 蛋白激酶C和钙调蛋白。 单通道电流研究 将允许钾的确定性表征（和 可能是这些细胞中存在的Cl）通道。

The goal of these studies is to identify the ionic currents that underlie salivary gland ductal function and to elucidate the intracellular factors that regulate these currents. The approach .Is to use the electrophysiological technique of patch clamping in order to complement and extend findings made using radiotracer techniques. The hypotheses to be tested are derived from results obtained in our preliminary studies, and from previous electrophysiological studies of salivary gland acinar cells, and generally test whether the same ionic currents and current regulating mechanisms present in acinar cells are also present in ductal cells. The studies utilize whole-cell current measurements from unstimulated and muscarinically stimulated HSG-PA tissue culture cells, and single channel current measurements on excised membrane patches from these cells. The whole-cell current studies allow analysis and characterization of resting cell membrane conduc- tances as well as effects of various intracellular agents involved in cell signaling such as G-proteins, IP3, Ca ion concentrations, protein kinase C and calmodulin. The single channel current studies will allow a definitive characterization of the potassium (and possibly the Cl) channels present in these cells.