ANTIBODY TO GLUCOSYLTRANSFERASE IN ADULTS AND CHILDREN

对唾液免疫应答重要的因子，其可以有效地改变 致病性微生物群的定殖和/或毒力包括个体发育的 发育，亚类限制，抗原给药途径，抗原 介绍和回忆。 在婴儿时期， 免疫系统面临着日益复杂的感染性挑战 可能致病的口腔植物群。 在以后的生活中， 对于保护性分泌免疫应答来说， 在微环境中表达分泌免疫的独特系统 整个口腔，即小唾液腺网络。 这 建议将探讨，在婴儿和幼儿， 分泌性免疫系统通过其与固有免疫系统相互作用 通过以下方式对微生物群进行分析：1）比较唾液抗体的发展， 经口感染的ELISA-Ab中的经口链球菌抗原 链球菌; 2）唾液进行性复杂性的测量 免疫印迹法检测婴儿自身口腔植物群表位的抗体应答 印迹法; 3）初始唾液的个体发育测量的鉴定 对B.百日咳抗原注射的抗体应答和回忆 对注射（破伤风类毒素）和口服（脊髓灰质炎病毒）的反应 疫苗，在ELISA-Ab。 还将探讨的潜力， 唾液免疫在小（唇）唾液腺唾液（LS）中的表达 5)用ELISA -IG法检测儿童LS中IgG和伊加的浓度， 青少年，年轻人和老年人; 6）测量的独特性 LS伊加和IgG同种型和亚类抗体，使用a）Western印迹 LS、腮腺和血清抗体对一系列口服抗原的分析， 和B）抗体谱型的等电聚焦/蛋白质印迹分析 7）探索刺激gLS的潜力 伊加和IgG抗体和分泌抗体的外周血单核细胞 用破伤风类毒素局部或全身免疫，和8） 通过结合全身性免疫增强LS抗体应答的可能性 局部应用抗原免疫。 这些研究应a） 提供关于唾液免疫的个体发生的新的基本信息， B）鉴定在初始免疫过程中具有免疫原性的毒力抗原， 殖民时期，因此可以纳入牙科 龋齿疫苗，c）有助于阐明小唾液腺的作用 网络在口服免疫，和d）确定的潜力， 通过免疫增强LS免疫力。

Factors important to salivary immune responses which can effectively modify colonization and/or virulence of pathogenic microbiota include ontogenic development, subclass restriction, route of antigen administration, antigen presentation, and anamnesis. In the infant a still-maturing secretory immune system is confronted with increasingly complex infectious challenges by potentially pathogenic oral flora. Later in life the pathways available for protective secretory immune responses are more varied and include a unique system for expression of secretory immunity in microenvironments throughout the oral cavity, namely, the minor salivary gland network. This proposal will explore, in infants and young children, the development of the secretory immune system through its interaction with indigenous microbiota by: 1) comparison of the development of salivary antibody to oral streptococcal antigens in ELISA-Ab with infection by oral streptococci; 2) measurement of the progressive complexity of salivary antibody responses to epitopes from an infant's own oral flora by Western blotting; 3) identification of the ontogeny measurement of initial salivary antibody response to injection of B.pertussis antigens and anamnestic responses to injected (tetanus toxoid) and orally administered (poliovirus) vaccines, in ELISA-Ab. Also explored will be the potential for mature salivary immune expression in minor (labial) salivary gland saliva (LS) by 5) identifying IgG and IgA concentration by ELISA -Ig in LS from children, adolescents, young and older adults; 6) measuring the distinctiveness of LS IgA and IgG isotypic and subclass antibody, using a) Western blot analysis of LS, parotid and serum antibody to a battery of oral antigens, and b) isoelectric focusing/Western blot analysis of antibody spectrotypes to individual antigens; and 7) exploring the potential for stimulation gLS IgA and IgG antibody and antibody-secreting peripheral blood monocytes by topical or systemic immunization with tetanus toxoid, and 8) the possibility for enhancing LS antibody responses by combining systemic immunization with local application of antigen. These studies should a) provide new basic information about the ontogeny of salivary immunity, b)identify virulence antigens which are immunogenic during the initial colonization period and which thus could be incorporated into a dental caries vaccine, c) help to clarify the role of the minor salivary gland network in oral immunity, and d) identify the potential for specific enhancement of LS immunity by immunization.