REGULATION OF MUCIN GLYCOPROTEIN BIOSYNTHESIS

这项调查的目的是研究调控机制 粘蛋白糖蛋白的生物合成和细胞加工。在……里面 特别是，我们有兴趣研究这些过程是如何 受正常生理发育的影响。 转录指导这些粘蛋白合成的信使核糖核酸 将通过分析这些特定信使的生物合成来研究 并通过与适当的cDNA杂交来定量它们的水平 探测器。 粘蛋白mRNA的翻译过程将通过免疫化学方法进行评估 用抗血清定量测定新合成的多肽(脱脂粘蛋白) 已知的对多肽核心的特异性。 最后，对脱辅酶粘蛋白的翻译后修饰进行了研究。 首先，我们将确定脱脂粘蛋白是否通过信号序列合成 存在(即：脱细胞粘蛋白前)。此外，我们还将尝试 确定影响结构和构象的决定因素 脱脂粘蛋白的糖基化。最后，我们将量化 特定的低聚糖并绘制它们在肽骨架上的位置， 以确定这些参数是否随正常、生理 发展。

The purpose of this investigation is to study the mechanisms which regulate the biosynthesis and cellular processing of mucin-glycoproteins. In particular, we are interested in examining how these processes are influenced by normal physiological development. The transcription of the mRNA which directs the synthesis of these mucins will be studied by analyzing the biosynthesis of these specific messengers and quantitating their levels by hybridization to the appropriate cDNA probes. The course of mucin mRNA translation will be assessed by immunochemical quantitation of the newly synthesized peptide (apo-mucin) using antiserum of known specificity to the peptide core. Lastly, post-translational modification of the apo-mucins will be studied. First we will determine if apo-mucin is synthesized with a signal sequence is present (i.e.: pre-apo-mucin). In addition, we will attempt to identify the structural and conformational determinants which influence glycosylation of the apo-mucin. Finally, we will quantitate the levels of specific oligosaccharides and map their position on the peptide backbone, to determine if these parameters vary with normal, physiological development.