MECHANISM OF ACTION OF STEROID HORMONES

这项研究的长期目标是了解分子和 参与糖皮质激素作用的代谢事件， 它们的靶细胞。 这包括阐明细胞内 调节机制，在生理上起作用，以介导 细胞代谢和遗传表达作为理解 内分泌调节叠加的分子机制。 这些努力将主要针对通过以下途径分析各种机制： 其中糖皮质激素在以下水平改变蛋白质生物合成的速率： 翻译. 目标之一将是严格审查 假设一些晚期（激素暴露2小时后）抑制性 糖皮质激素对转运和合成反应的影响， 包括蛋白质生物合成，是细胞对蛋白质合成的反应。 糖皮质激素诱导的细胞ATP生成抑制， 导致腺嘌呤核苷酸比例的小的稳态变化。 对糖皮质激素致死作用的研究也在进行中， 一组新的蛋白质的出现，证实了细胞对 糖皮质激素诱导的细胞杀伤。

The long-term objectives of this research are to understand the molecular and metabolic events that are involved in the actions of glucocorticoid hormones on their target cells. This includes the elucidation of the intracellular regulatory mechanisms that operate physiologically to mediate changes in cellular metabolism and genetic expression as a background for understanding of the molecular mechanisms through which endocrine regulation is superimposed. The efforts will largely be directed to an analysis of the mechanisms through which glucocorticoids alter rates of protein biosynthesis at the level of translation. One of the objectives will be a rigorous examination of a hypothesis that some of the late (after 2 hrs of hormone exposure) inhibitory effects of glucocorticoid hormones on transport and synthetic reactions, including protein biosynthesis, are the cells' response to a glucocorticoid-induced inhibition of cellular ATP production, which in turn leads to small, steady-state changes in ratios of adenine nucleotides. Studies on the lethal actions of glucocorticoids are also underway which focus on the appearance of a novel set of proteins that confirm cellular resistance to glucocorticoid-induced cell killing.