BIOCHEMICAL ASPECTS OF RENAL ELECTROLYTE TRANSPORT

本提案是关于药物在肾脏中分布的研究。 的行动 药物对肾脏的影响以及药物相互作用。 这些子项目是：1）一项研究 药物的主动重吸收机制。 所涉及的问题是： 在不同的药物之间是否能证明转运的相互抑制作用 哪些被积极地重吸收？这种重吸收机制是否与 尿酸是什么？主动重吸收通量是否可以被证明为 在清除实验中似乎不被重吸收的促尿酸排泄药物？ 2)唑胺的促尿酸排泄作用。 问题是：有机食品 碱本身是促尿酸的，或者它必须在体内转化为阴离子 代谢物。 3)氯噻嗪的降钙作用。 问题： 低钙作用是酶抑制的结果 磷酸二酯酶？ 4)一种碳酸酐酶的大分子抑制剂。 的 问：腔内碳酸酐酶在肿瘤中的定量作用是什么？ 重碳酸盐的重吸收 实验将在狗、黑猩猩、Cebus猴和大鼠中进行。 主要技术有肾清除术、停流术、显微穿刺术， 微灌注

This proposal is for the study of the renal disposition of drugs. The actions of drugs on the kidney, and drug interactions. The subprojects are: 1) A study of the active reabsorptive mechanism(s) for drugs. The questions addressed are: Can mutual inhibition of transport be demonstrated among the various drugs which are actively reabsorbed? Is this reabsorptive mechanism the same as the mechanism for uric acid? Can active reabsorptive fluxes be demonstrated for uricosuric drugs which do not seem to be reabsorbed in clearance experiments? 2) The uricosuric action of zoxazolamine. The question is: Can an organic base per se be uricosuric or must it be converted in vivo to an anionic metabolite. 3) The hypocalicuric action of chlorothiazide. The question: Is the hypocalcuric action a result of inhibition of the enzyme(s) phosphodiesterase? 4) A macromolecular inhibitor of carbonic anhydrase. The question: What is the quantitative role of lumenal carbonic anhydrase in the reabsorption of bicarbonate? The experiments will be conducted in dogs, chimpanzees, Cebus monkeys and rats. The major techniques are renal clearance, stop-flow, micropuncture, microperfusion.