RENAL GLUTATHIONE METABOLISM: GLUTAMYLTRANSPEPTIDASE

谷胱甘肽的分解代谢通过器官间过程发生，该过程 包括从各种组织中释放，通过血浆和 主要发生在肾脏的水解物。肾提取液 一次通过80-95%的血浆谷胱甘肽。肾组织分解代谢 谷胱甘肽是由γ-谷氨酰转肽酶和以下任一种 也与管腔表面相关的两种肽酶活性 肾刷状缘膜。肾脏的快速周转 谷胱甘肽涉及其从近端小管细胞的初始分泌 进入管腔。这项提议的具体目的是进一步 鉴定γ-谷氨酰转肽酶的疏水结构域 确定这两种多肽酶活性对 半胱氨酸甘氨酸的水解，以表征肾脏的作用机制 谷胱甘肽的提取及其定量研究 分泌物，并探讨其周转的生理功能 肾脏谷胱甘肽。

The catabolism of glutathione occurs through an inter-organ process which involves release from various tissues, transport via the plasma and hydrolysis which occurs primarily in the kidney. The kidney extracts 80-95% of the plasma glutathione in a single pass. Renal catabolism of glutathione is carried out by Gamma-glutamyltranspeptidase and either of two peptidase activities that are also associated with the lumenal surface of the renal brush border membrane. The rapid turnover of renal gluthathione involves its initial secretion from the proximal tubbule cells into the tubular lumen. The specific aims of this proposal are to further characterize the hydrophobic domain of the Gamma-glutamyltranspeptidase, to determine the relative contribution of the two peptidase activities to the hydrolysis of cysteinylglycine, to characterize the mechanism of renal extraction of glutathione, to quantitate the process of glutathione secretion and to investigate the physiological function of the turnover of renal glutathione.