Production of difficult to express essential bacterial proteins

AbSynth Biologics(http://www.absynthbiologics.co.uk))为预防细菌感染提供了一个新的蛋白质抗原平台。Absynth的方法是一种通过预防感染从而直接减少抗生素使用来对抗抗生素耐药性增加的手段。Absynth已经证明了其方法在原则上是可行的，但对Absynth来说，关键的一步是高效且具有成本效益地生产其蛋白质(或其变体)，以便能够以经济成本生产出质量一致的蛋白质。这就是曼彻斯特大学的专业知识非常重要的地方，并构成了这个翻转项目中共享课程的基础。曼彻斯特大学通过Eddie McKenzie博士和蛋白质表达便利性(通过在多个学术和工业合作项目中成功优化蛋白质表达测试的能力)积累的大量专业知识和技术技能，证明了开发生产Absynth蛋白质的高效工艺的知识和技术技能，这些蛋白质将进行测试，以进一步优化其在预防感染中的使用。该研究项目将看到来自AbSynth的蛋白质被用于曼彻斯特大学开发的独特的图谱技术。翻转开关科学家(Hirra Hussain女士)将在曼彻斯特大学(6-7个月)和Absynth的实验室(5-6个月)承担这项工作。所述方案提供了一种互惠互利的知识和技术交流(由一名将在两个地点工作的科学家纳入工作方案)。所述项目的成功将为Absynth扩展到其他项目以及曼彻斯特大学将其对基本生产机制的改进应用于其他相关分子提供一个示范方法。

Absynth Biologics (http://www.absynthbiologics.co.uk) has a novel platform of protein antigens for prevention of bacterial infections. Absynth's approach is a means of fighting the increase in antibiotic resistance by preventing infections and therefore directly reducing antibiotic use. Having already demonstrated its approach can work in principle, a key step for Absynth is the production of its proteins (or variants of them) efficiently and cost-effectively, so that they can be made to a consistent quality at an economic cost. This is where the expertise of the University of Manchester is important and forms the basis for the shared programme in this FLIP project. The University of Manchester, through the considerable expertise built up by Dr Eddie McKenzie and the Protein Expression Faciility (competencies that have been tested tested by successful optimsiation of expression of proteins in multiple academic and industrial collaborative project,) has proven knowledge and technical skills to develop efficient processes to produce the Absynth proteins, which will be tested to further optimize their use in preventing infection. The research project will see the proteins from Absynth being put through the unique profiling technology developed at the University of Manchester. The FLIP interchanger scientist (Ms Hirra Hussain) will undertake this work at both at the University of Manchester (6-7 months) and at Absynth's laboratories (5-6 months). The programme described presents a mutally beneficial exchange of knowledge and technologies (embedded into the work programme by a scientist who will work at both sites). Success in the project outlined will provide an exemplar approach for Absynth to expand into other projects and for the University of Manchester to apply its improved understanding of fundamental production mechanisms to other related molecules.