NA/ION REABSORPTION & H/ION SECRETION RENAL MECHANISMS

这项提议的长期目标是描述离子的特性 顶膜和基侧膜亚群中的运输系统 肾细胞的囊泡内细胞器。另一个长期目标 是确定调节细胞膜上离子运输的因素 水平。离子之间存在动态关系的假设 细胞膜和细胞内囊泡群中的转运蛋白 将会被检查。该项目的具体目标是：1.确定 并表征了Na+、H+、K+、C1-等的运输系统 肾细胞膜中的阴离子。2.评价活体实验的效果 调理(饮食、酸中毒、激素等)关于肾细胞活性的研究 膜运输系统。3.研究离子输运系统 在肾来源的连续细胞系中。4.确定所扮演的角色 转位素池在肾细胞调节中的作用 膜运输系统(“膜循环假说”)。5.至 评估次要位置的性质和调节作用(“修饰物 部位“)在离子运输系统上，可通过以下方式调节运输活动 动力机制。 细胞膜群体将通过差速离心法制备， 密度梯度分馏技术和逆流分布 在双水相体系中。膜种群将通过以下方式确定 电子标记酶的测定和形态研究 显微镜。离子输运研究将用标准同位素进行 跨膜pH和荧光检测技术及方法 电压梯度。 了解离子转运的机制和调节因素 这些系统将是推动整体 了解肾功能，以及定义适应性的性质 生理性扰动的变化。

The long term objective of this proposal is to characterize the ion transport systems in subpopulations of apical and basolateral membranes and of intravesicular organelles of renal cells. Another long term objective is to define the factors which regulate ion transport at the cell membrane level. The hypothesis that there is a dynamic relationship between ion transporters in the cell membrane and in intracellular vesicle populations will be examined. The specific aims of the project are: 1. To identify and characterize the transport systems for Na+, H+, K+, C1-, and other anions in renal cell membranes. 2. To evaluate the effects of in vivo conditioning (diet, acidosis, hormones, etc.) on the activity of renal cell membrane transport systems. 3. To investigate the ion transport systems in continuous cell lines of renal origin. 4. To determine the role played by intracellular pools of transpoerrts in the regulation of renal cell membrane transport systems ("membrane recycling hypothesis"). 5. To evaluate the nature and regulatory role of secondary sites ("modifier sites") on ion transport systems which may regulate transport activity by kinetic mechanisms. Cell membrane populations will be prepared by differential centrifugation, density gradient fractionation techniques, and counter-current distribution in aqueous 2-phase systems. Membrane populations will be identified by marker enzyme determinations and morphologic studies with an electron microscope. Ion transport studies will be done with standard isotopic techniques and fluorescence methods for measuring transmembrane pH and voltage gradients. Understanding the mechanisms of ion transport and factors which regulate these systems will be an important step in furthering the overall understanding of renal function, as well as defining the nature of adaptive changes to physiologic pertubations.