RENAL GLUTATHIONE METABOLISM: GLUTAMYLTRANSPEPTIDASE

谷胱甘肽的催化作用是通过一个器官间过程发生的， 包括从各种组织中释放，通过血浆转运， 水解主要发生在肾脏。 肾脏提取物 单次通过80-95%的血浆谷胱甘肽。 肾性卡他 谷胱甘肽是由γ-谷氨酰转肽酶进行的， 两种肽酶活性也与内腔表面有关 肾刷状缘膜的厚度 肾脏的快速周转 谷氨酰胺包括其从近端小管细胞的初始分泌 进入管状管腔。 这项建议的具体目的是进一步 表征γ-谷氨酰转肽酶的疏水结构域， 确定两种肽酶活性对酶活性的相对贡献。 水解半胱氨酰甘氨酸，以表征肾 谷胱甘肽的提取，以定量谷胱甘肽的过程 分泌和研究周转的生理功能， 肾脏谷胱甘肽

The catabolism of glutathione occurs through an inter-organ process which involves release from various tissues, transport via the plasma and hydrolysis which occurs primarily in the kidney. The kidney extracts 80-95% of the plasma glutathione in a single pass. Renal catabolism of glutathione is carried out by Gamma-glutamyltranspeptidase and either of two peptidase activities that are also associated with the lumenal surface of the renal brush border membrane. The rapid turnover of renal gluthathione involves its initial secretion from the proximal tubbule cells into the tubular lumen. The specific aims of this proposal are to further characterize the hydrophobic domain of the Gamma-glutamyltranspeptidase, to determine the relative contribution of the two peptidase activities to the hydrolysis of cysteinylglycine, to characterize the mechanism of renal extraction of glutathione, to quantitate the process of glutathione secretion and to investigate the physiological function of the turnover of renal glutathione.