RAMAN AND TIME-RESOLVED RAMAN STUDIES OF HEME PROTEINS

血红素蛋白参与了许多重要的生理过程。 过程，包括：向组织输送氧气；衍生 营养物质氧化所储存的能量；防护性破坏 潜在危险的过氧化物质与有毒物质的活化和消除 污染物和致癌物。尽管这一非凡的功能 多样性，所有这些物种都利用血红素族作为活跃的复合体。 庞南特。确定造成这种差异的因素 函数的作用对于理解 正常和异常状态下的行为。 这项研究计划的长期目标是阐明 这些不同蛋白质相互作用的分子机制 能有效调节其反应性的普通亚铁血红素基团。按顺序 为了阐明功能行为的分子基础，许多 强大的光谱技术被用来探测关键的相互作用 在血红素和蛋白质之间-并确定微妙的效果 电子结构和反应活性的结构微扰 蛋白质中的活性部位和其他关键部位。校长 在这一阶段的工作中将采用的光谱方法包括 共振拉曼和时间分辨共振拉曼(在可见光中 和紫外线区域)，其利用新的快速混合装置来 为短暂的中间体提供结构信息。 这种方法适用于天然和系统修饰的蛋白质。 代表了一种现实的尝试，以阐明重要的分子 控制机制导致了如此显著的功能多样性。

Heme proteins are involved in a large number of physiologically important processes, including: delivery of oxygen to the tissues; derivation of stored energy from oxidation of nutrients; protective destruction of potentially dangerous peroxides and activation and elimination of toxic pollutants and carcinogens. Despite this remarkable functional diversity, all of these species employ the heme group as the active com- ponent. Identification of the factors responsible for this varied function would be of obvious benefit for attaining an understanding of the behavior in normal and abnormal states. The long term objective of this research program is the elucidation of the molecular mechanisms by which these various proteins interact with the common heme group to effectively regulate its reactivity. In order to clarify the molecular basis of functional behavior, a number of powerful spectroscopic techniques are employed to probe key interactions between the heme and the protein-and to determine the effect of subtle structural perturbations on the electronic structure and reactivity of the active sites and other key sites within-the protein. The principal spectroscopic methods to be employed during this phase of the work are resonance Raman and time-resolved resonance Raman (in both the visible and ultraviolet regions) which exploit novel rapid-mixing devices to yield structural information for short-lived intermediates. This approach applied to native and systematically modified proteins represents a realistic attempt to elucidate the important molecular control mechanisms responsible for such remarkable functional diversity.