POLYAMINES IN THE REGULATION OF GASTROINTESTINAL GROWTH

这项提案的总体目标是研究和确定 腐胺、亚精胺、精胺和身体中的多胺 多胺类化合物腐胺、亚精胺、精胺和身体碱在人体内的作用 胃肠生长的调节。肠道的生长将是 在体内和体外条件下进行了各种实验研究 诱导胃肠道生长的干预措施。美国政府的角色 多胺将根据以下提供的大纲进行研究 具体目标：1.研究和确定管腔内的作用 原核和真核来源的多胺作为蛋白质的调节剂 粘膜生长。2.胃肠极化的研究 上皮细胞和多胺在这些细胞中的矢量运动。 3.研究各种生长因子的营养效应及其作用 与胃肠道多胺依赖生长的关系。 理解这一过程的规则的重要性在于 强调的是意识到胃肠道的许多疾病 事实上，肠道是粘膜生长的障碍。食道反流， 慢性胃炎，佐林格-埃里森增殖性粘膜。这个 多胺是一组普遍分布的聚阳离子，它们是 正常生长和分化所必需的；这项工作的目标是 目的：阐明多胺在粘膜生长中的作用。 建议研究从以下来源的多胺的可用性 无菌和无菌大鼠结肠微花多胺代谢。 已经设计了实验来研究腐胺的直接影响。 刺激十二指肠隐窝细胞系(IEC-6)合成DNA的实验研究 文化。IEC-6细胞系也将被用于研究多胺 从该细胞的顶端和基底侧面摄取。这场运动 多胺进入胃肠道上皮细胞的情况将在整体上得到证实 动物。最后，提出了相关的生长促进效应。 不同营养因子对多胺的调节能力 新陈代谢。这些实验将被用来确定 多胺是粘膜刺激过程中的一种常见介质 或者，如果它们只起到一个允许的作用。

The overall objective of this proposal is to study and define the role of the polyamines putrescine, spermidine, spermine and cadaverine in the role of the polyamines putrescine, spermidine, spermine and cadaverine in the regulation of gastrointestinal growth. Growth of the gut will be studied under in vivo and in vitro conditions after various experimental interventions which induce gastrointestinal growth. The role of the polyamines will be studied under the outline provided by the following specific aims: 1. To study and define the role of intraluminal polyamines for prokaryotic and eukaryotic sources as regulators of mucosal growth. 2. To study the polarization of gastrointestinal epithelial cell and the vectorial movement of polyamines in these cells. 3. To study the trophic effects of various growth factors and their relationship to polyamine-dependent growth in the gastrointestinal tract. The importance of understanding the regulation of this process is underscored by the realization that many diseases of the gastrointestinal tract are, in fact, disorders of mucosal growth. Esophageal reflux, chronic gastritis, Zollinger-Ellison hyperproliferative mucosa. The polyamines are a group of ubiquitously distributed polycations which are required for normal growth and differentiation; the goal of this work is to elucidate the role of the polyamines during mucosal growth. It is proposed to study the availability of polyamines derived from colonic microfloral polyamine metabolism in gnotobiotic and axenic rats. Experiments have been designed to study the direct effects of putrescine to stimulate DNA synthesis in a duodenal crypt cell line (IEC-6) in culture. The IEC-6 cell line will also be used to study polyamine uptake from both apical and basolateral sides of this cell. The movement of polyamines into the GI epithelial cell will be confirmed in the whole animal. Finally, it is proposed to relate the growth promoting effects of various trophic factors to their ability to modulate polyamine metabolism. These experiments will be used to determine whether polyamines serve as a common mediator during stimulation of mucosal growth or if they serve only a permissive role.