STUDIES UPON THE OCULAR LENS

I. 本项目的目的是阐明 氧化引起白内障。 我们将使用H2 O2模型系统并研究 对Na/K ATP酶和其他转运系统影响。 一般代谢 蛋白质合成，蛋白质和脂质结构，蛋白质钙 还将检查相互作用和细胞形态。 其他模型 用于氧化损伤的系统，例如光解和高O2张力，将 也被研究。 二. 将研究人类白内障，以将发现与模型相关联 系统和追求其他问题，如二硫化物和蛋氨酸 亚砜还原，水不溶性和高分子量蛋白质， 与高分子量蛋白质复合物的基质-膜相互作用， 与光解和多胺有关的共价交联，蛋白水解， 磷酸化、H2 O2来源以及与抗坏血酸的关系。 三. 利用模型系统进行药物干预。

I. The aims of this project are to delineate the mechanism by which oxidation causes cataract. We shall use the H2O2 model system and study its effect on Na/K ATPase and other transport systems. General metabolism and protein synthesis, protein and lipid structure, proteincalcium interaction, and cell morphology will also be examined. Other model systems for oxidative insult, such as photolysis and high O2 tension, will also be studied. II. Human cataract will be investigated to correlate findings with model systems and to pursue other problems such as disulfide and methionine sulfoxide reduction, the water insoluble and high molecular weight protein, matrix-membrane interactions with high molecular weight protein complexes, covalent crosslinking related to photolysis and polyamines, proteolysis, phosphorylation, H2O2 origin and relationship to ascorbate. III. Drug intervention utilizing model systems.