BIOCHEMISTRY AND PHARMACOLOGY OF THE RETINA

这是一项研究视网膜生物化学和药理学的建议。 特别强调环核苷酸。总体目标是定义 环磷酸腺苷在视网膜功能和代谢中的作用我们将解决和 探索视网膜包含循环AMP和循环GMP系统的假设， 每个都在不同的亚蜂窝或蜂窝位置，并且每个都有不同的 特点和功能。利用定量组织化学方法和 我们计划识别、定位和表征鸟苷环化酶的技术， 腺苷酸环化酶、环核苷酸磷酸二酯酶和环状 视网膜中的核苷酸依赖蛋白激酶。区域嘌呤核苷酸 视网膜的新陈代谢也将被评估。此外，我们希望定义 什么因素(即光、神经递质、离子、细胞去极化) 调节视网膜中的环核苷酸水平并解释其机制。这 将视网膜生物化学和形态学联系起来的项目应该会有所帮助 解释视觉背后的一些分子机制。

This is a proposal to study biochemistry and pharmacology of the retina with particular emphasis on cyclic nucleotides. The overall aim is to define the role of cyclic AMP in retinal function and metabolism. We will address and explore the hypothesis that retina contains cyclic AMP and cyclic GMP systems, each in different subcellular or cellular locations and each with different characteristics and functions. Utilizing quantitative histochemical methods and techniques we plan to identify, localize and characterize guanylate cyclases, adenylate cyclases, cyclic nucleotic phosphodiesterases and cyclic nucleotide-dependent protein kinases in retina. Regional purine nucleotide metabolism in retina also will be evaluated. In addition, we wish to define what factors (i.e., light, neurotransmitters, ions, cellular depolarization) regulate cyclic nucleotide levels in retina and explain their mechanisms. This project, which correlates retinal biochemistry and morphology, should help explain some molecular mechanisms underlying vision.