STUDIES UPON THE OCULAR LENS

这一项目的目的是描述 氧化会导致白内障。我们将使用H_2O_2模型系统进行研究 它对Na/K-ATPase等转运系统的影响。一般代谢 和蛋白质合成，蛋白质和脂肪结构，蛋白质钙 相互作用，以及细胞形态也将被检查。其他型号 氧化损伤的系统，如光解和高氧压，将 也有待研究。 II.将对人类白内障进行调查，以将研究结果与模型相关联 并寻求其他问题，如二硫化物和蛋氨酸 亚砜还原，水不溶性和高分子量蛋白质， 基质-膜与高分子量蛋白质复合体的相互作用， 与光解和多胺有关的共价交联，蛋白质降解， 磷酸化，过氧化氢的来源及其与抗坏血酸的关系。 利用模式系统进行药物干预。

I. The aims of this project are to delineate the mechanism by which oxidation causes cataract. We shall use the H2O2 model system and study its effect on Na/K ATPase and other transport systems. General metabolism and protein synthesis, protein and lipid structure, proteincalcium interaction, and cell morphology will also be examined. Other model systems for oxidative insult, such as photolysis and high O2 tension, will also be studied. II. Human cataract will be investigated to correlate findings with model systems and to pursue other problems such as disulfide and methionine sulfoxide reduction, the water insoluble and high molecular weight protein, matrix-membrane interactions with high molecular weight protein complexes, covalent crosslinking related to photolysis and polyamines, proteolysis, phosphorylation, H2O2 origin and relationship to ascorbate. III. Drug intervention utilizing model systems.