NEURAL RETINA RECOGNITION PROTEIN

多细胞动物的生化和生理过程需要 许多细胞之间的相互协调作用。这些电池的维护-电池 由它们产生的特定细胞连接的关联是 组织功能的基础。本项目的目的是确定 一种特定的胚胎细胞表面成分在控制血吸虫感染中的作用 视网膜细胞-细胞识别和神经细胞的发育 关系。我们已经提纯了这种成分，神经视网膜科尼丁； 它是一种糖蛋白，可以增强分离的雏鸡的重新聚集 和体外培养的小鼠视网膜神经细胞。扫描电子显微镜 细胞表面相关蛋白的定位表明，其在 视网膜，在不同的视网膜细胞类型上，以及在这样的 在发育过程中的分布，可能对阐明其作用具有重要意义 活体内的干邑香豆素。我们建议，首先，识别其他细胞表面 这些分子必须与科尔马素相互作用，从而重建 细胞-细胞识别机制。这将通过亲和力来实现 通过层析来鉴定与科尔马素结合的分子，并通过研究一种 制备膜囊体外相互作用的简易模型系统 来自视网膜细胞。我们还将利用可用的多行式 用考马菌素抗体确定考马菌素在体内的定位。这 将与分离的细胞类型上的考马素的分布进行比较 视网膜通过密度梯度分级，以确定 视黄素在视网膜两个关键形态发生阶段的可能作用 发展。这些都是；视网膜之间的早期诱导相互作用 和其他视区组织，并发展成层状 成熟视网膜的结构。最后，我们建议测试一个特定的 科尼金在后来的突触发育中发挥作用的假说 视网膜发生。

Biochemical and physiological processes in multicellular animals require the coordinated interactions of many cells. Maintenance of these cell-cell associations of the specific cell junctions which arise from them is the basis of tissue function. The purpose of this Project is to determine the role of one specific embryonic cell surface constituent in the control of retina cell-cell recognition, and in the development of neuronal connections. We have purified this constituent, the neural retina cognin; it is a glycoprotein which enhances the reaggregation of dissociated chick and mouse neural retina cells in vitro. Scanning electron microscopy localization of the cell surface cognin suggests that its distribution in the retina, on differnt retina cell types, and the changes in such a distribution during development, may be significant in elucidating the role of cognin in vivo. We propose, first, to identify other cell surface molecules which must interact with cognin, and to thereby reconstruct the mechanism of cell-cell recognition. This will be done by affinity chromatography to identify molecules which bind cognin, and by studying a simple model system, the in vitro interaction of membranevesicles prepared from retina cells. We will also utilize the available polydeterminant antibody to cognin to determine the localization of cognin in vivo. This will be compared to the distribution of cognin on cell types isolated from the retina by density gradient fractionation in order to determine the possible role of cognin in two critical morphogenetic phases of retina development. These are; the early inductive interactions between retina and other optic area tissues, and the development of the stratified architecture of the mature retina. Finally, we propose to test a specific hypothesis that cognin plays a role in the development of synapses later retinogenesis.