NEURAL AND PHARMACOLOGICAL CONTROLS OF AQUEOUS DYNAMICS

这项提案的目的是研究神经和药理学 控制房水动力学。 神经机制将研究 神经肽（胰多肽、血管活性肽）的眼部作用 肠多肽等）和生理调节剂 动力学 这些药物将通过外周（局部， 前房内、玻璃体内）和中央（第三脑室和下丘脑 显微注射）给药途径。 神经通路将被 通过消融上级颈神经节、更大 岩浅神经和三叉神经。 药理学控制 将研究眼部碳酸酐酶，特别注意局部 活性抑制剂;眼内压降低作用 局部多巴胺以及特异性多巴胺受体（DA 1）的作用 和DA 2）激动剂和拮抗剂;以及 局部大麻素化合物。 房水动力学将包括 眼内压，流出道，巩膜外静脉压，房水 体液流量以及眼血流量和体积。 这些研究涉及到 调节眼内压。 结果将提供信息 神经肽作为房水生理调节剂的作用 动力学，并可能提供线索的基础病理生理学 青光眼 眼内压的药理学调节将提供 对正常房水生理学和可能的新青光眼的认识 治疗剂。

The objectives of this proposal are to study neural and pharmacological controls of aqueous humor dynamics. Neural mechanisms will investigate the ocular effects of neuropeptides (pancreatic polypeptide, vasoactive intestinal polypeptide, etc.) and physiological regulators of aqueous humor dynamics. These agents will be studied via peripheral (topical, intracameral, intravitreal) and central (third ventricle and hypothalamic microinjection) routes of administration. Neural pathways are to be investigated by ablation of the superior cervical ganglion, greater superficial petrosal nerve and trigeminal nerve. Pharmacological controls will study ocular carbonic anhydrase with special attention to topically active inhibitory agents; the intraocular pressure lowering effect of topical dopamine as well as the effect of specific dopamine receptor (DA1 and DA2) agonists and antagonists; and the intraocular pressure effect of topical cannabinoid compounds. Aqueous humor dynamics will include intraocular pressure, outflow facility, episcleral venous pressure, aqueous humor flow, and ocular blood flow and volume. These studies relate to the regulation of intraocular pressure. The results will provide information on the role of neuropeptides as physiological regulators of aqueous humor dynamics and may provide clues about the underlying pathophysiology of glaucoma. Pharmacological modulation of intraocular pressure will provide insight into normal aqueous humor physiology and possibly new glaucoma therapeutic agents.