ELECTROPHYSIOLOGY OF CILIARY BODY TRANSPORT PROCESSES

房水形成主要是超滤（透析）的复合物 在存在静水压力的情况下）和由 睫状体突起的上皮。 最终房水 组成是由这两种行为决定的，这两种行为受到 全身和局部代谢、体液、循环或神经因素。 本建议的目的是调查运输过程中hte 分离虹膜睫状体独立于这些因素。 研究将是 使用来自白化病和有色兔、猫、猴和 人类尸体的眼睛 实验技术将包括以下内容：1） 组织电位差的跨上皮测量，短路 电流、离子通量和流体输运，以提供 运输过程。 2)氧消耗研究的孤立条 睫状突以测量组织的代谢状态，以及 药物影响主动转运速率的机制。 第三章 常规、离子特异性和pH微电极研究， 离子移位穿过双上皮层的步骤 睫状突 体外跨上皮和细胞内电测量可以 提供了重要的见解，正常的生理和运输， 上皮渗漏。 代谢参数的表征允许 影响药物作用的特异性评价 跨上皮和细胞内电参数。 药理 研究可以提供虹膜睫状体的基本和改变状态的信息， 身体分泌和运输过程。 需要调查的特工包括 (Na+，K+）ATP酶抑制剂，碳酸酐酶抑制剂，离子交换 抑制剂、肾上腺素能激动剂和拮抗剂。

Aqueous humor formation is mainly a composite of ultrafiltration (dialysis in the presence of hydrostatic pressure) and active secretion by the epithelium of the processes of the ciliary body. Final aqueous humor composition is determined by both of these actions which are influenced by systemic and local metabolic, humoral, circulatory or neurologic factors. The aim of this proposal is to investigate transport processes in hte isolated iris-ciliary body independent of these factors. Studies are to be performed using tissue from albino and pigmented rabbits, cats, monkeys and human autopsy eyes. Experimental technique will include the following: 1) Transepithelial measurement of tissue potential difference, short-circuit current, ionic fluxes and fluid transport to provide an overview of active transport processes. 2) Oxygen consumption studies on isolated strips of ciliary processes to measure the metabolic status of the tissue as well as the mechanism by which agents affect rates of active transport. 3) Conventional, ion specific and pH microelectrode studies to characterize the steps in ion translocation across the double epithelial layer of the ciliary processes. In vitro transepithelial and intracellular electrical measurements can provide important insights into the normal physiology and transport of this leaky epithelium. Characterization of the metabolic parameter permits evaluation of the specificity of action for drugs which affect transepithelial and intracellular electrical parameters. Pharmacological studies can give information on basic and altered states of iris-ciliary body secretory and transport processes. Agents to be investigated include (Na+,K+) ATPase inhibitors, carbonic anhydrase inhibitors, ion exchange inhibitors, adrenergic agonists and antagonists.