PROTECTIVE MECHANISM OF HERPES SIMPLEX VIRUS ANTIBODY

单纯疱疹病毒(HSV)是一种重要的眼部病原体。 天哪。感染后的细胞和体液免疫 引起了人们的回应。然而，相对的贡献是 每一项都对限制或加剧病毒感染做出了贡献 人们对此知之甚少。我们和其他调查人员已经证明 单纯疱疹病毒特异性单抗的被动转移将 使宿主能够抵抗致命挑战剂量的病毒。更多 最近发现，单纯疱疹病毒的特异性mcAbs 角膜感染后24小时给药的糖蛋白能够 预防或消除间质疾病的发展和 促进HSV眼部感染的消退。 使用小鼠眼部感染模型，我们建议研究 抗体如何控制HSV复制的基本方面。 针对单纯疱疹病毒糖蛋白、gD和选定的其他的单抗 糖蛋白，将被提纯，表征，并测试其 抑制HSV复制和促进病毒清除的能力 从BALB/c小鼠的角膜组织中提取。在进行这项工作时 工作，将特别强调评估 免疫球蛋白同型和表位特异性的重要性。一个 主要目的是研究抗体(S)通过 才能发挥其保护作用。需要检验的假设是 抗体依赖的细胞毒性是一种重要的 体内的防御机制。 从这些研究中获得的信息将确定属性 抗HSV抗体必须具备，才能最佳地保护 并为体液免疫的作用提供了新的见解 应对措施在遏制病毒感染方面发挥了作用。

Herpes simplex virus (HSV) is an important ocular pathogen in man. Following infection both cellular and humoral immune responses are elicited. However, the relative contribution that each makes toward limiting or exacerbating virus infection is poorly understood. We and other investigators have shown that passive transfer of HSV-specific monoclonal antibody (mcAb) will enable the host to resist a lethal challenge dose of virus. More recently, it has been found that mcAbs specific for HSV glycoproteins given 24 hours after corneal infection are able to prevent or ameloriate development of stromal disease and promote resolution of HSV eye infection. Using a murine ocular infection model, we propose to investigate basic aspects of how antibody can control HSV replication. McAbs specific for HSV glycoproteins, gD, and selected other glycoproteins, will be purified, characterized, and tested for their capacity to inhibit HSV replication and promote virus clearance from corneal tissue of the BALB/c mouse. In conducting this work, special emphasis will be placed on evaluating the importance of immunoglobulin isotype and epitope specificity. A primary goal is to investigate the mechanism(s) by which antibody can exert its protective effect. The hypothesis to be tested is that antibody-dependent cellular cytotoxicity is an important defense mechanism in vivo. The information gained from these studies will identify properties anti-HSV antibodies must have in order to protect optimally in vivo, and provide new insights into the role the humoral immune response plays in containing virus infection.