ELECTROPHYSIOLOGY OF CILIARY BODY TRANSPORT PROCESSES

眼房水的形成主要是由眼内分泌物的主动分泌组成的。 双层睫状突上皮（内部无色素，外部 色素上皮）和超滤（在存在 流体静压）。 最终的房水组成由以下测定： 这两种行为都受到许多因素的影响， 全身和局部代谢改变、体液影响、循环 变化和神经系统的改变。 这项建议的目的是 研究离体虹膜睫状体（I-CB）的转运过程 独立于这些因素。 实验技术将包括 1）在组织的I-CB制备中的跨上皮测量 电位差，短路电流，组织电阻，离子 通量和流体输运，以提供主动输运的概述 睫状上皮中的突起。 2)常规离子比和pH 微电极研究，以表征离子移位的步骤 穿过睫状突的双层上皮层。 第三章 非色素层细胞内膜的膜片钳研究。 体外跨上皮、细胞内微电极和膜片钳 微量吸管测量可以提供对正常生理的深入了解， 和睫状上皮的主动运输。 药理研究 将提供有关睫状体上皮细胞的基本和改变状态的信息， 分泌和运输过程。 需要调查的特工包括 肾上腺素能激动剂和拮抗剂，碳酸酐酶抑制剂，钙 通道阻断剂和神经肽（例如抗利尿激素， 血管活性肠多肽等）。 机制知识 参与积极生产的水状体，以及影响 药物对睫状上皮主动转运的影响， 将提供青光眼治疗方式的见解， 降低房水形成的速率。

Aqueous humor formation is mainly a composite of active secretion by the double layered ciliary process epithelium (inner nonpigmented and outer pigmented epithelium) and of ultrafiltration (dialysis in the presence of hydrostatic pressure). Final aqueous humor composition is determined by both of these actions which are influenced a number of factors including systemic and local metabolic alterations, humoral influences, circulatory changes and neurological modifications. The aim of this proposal is to investigate transport processes in the isolate iris-ciliary body (I-CB) independent of these factors. Experimental techniques will include the following 1) Transepithelial measurement in the I-CB preparation of tissue potential difference, short-circuit current, tissue resistance, ionic fluxes and fluid transport to provide an overview of active transport processes in the ciliary epithelium. 2) Conventional ionic specific and pH microelectrode studies to characterize the steps in ion translocation across the double epithelial layer of the ciliary processes. 3) Patch-clamp studies on the inner cell membrane of the nonpigmented layer. In virto transepithelial, intracellular microelectrode, and patch-clamp micropipette measurements can provide insights into the normal physiology and active transport of the ciliary epithelium. Pharmacological studies will provide information on basic and altered states of ciliary epithelial secretory and transport processes. Agents to be investigated include adrenergic agonists and antagonists, carbonic anhydrase inhibitors, calcium channel blocking agents, and neuropeptides (e.g. anti-diuretic hormone, vasoactive intestinal polypeptide, etc). Knowledge of the mechanisms involved in the active production of aqueous humor, as well as the effects of pharmacological agents on active transport by the ciliary epithelium, will provide insights into glaucoma therapeutic modalities directed to reducing the rate of aqueous humor formation.