THE TEAR FILM AND THE OCULAR SURFACE: VITAMIN A IN TEAR

拟议工作的总体目标是研究 泪液与眼表细胞的关系。它有 最近有报道称，视黄醇存在于泪水中。因为维生素A 可能影响并参与细胞的调节和成熟。 对于角膜和结膜，最初的研究将集中在交付 维生素A通过泪水进入眼表的假说 泪液患者的眼表疾病，尤其是角质化 膜异常的部分原因可能是维生素A的供应不足 通过泪水转移到眼表上皮。 该项目的具体目标是： 1)用高压液体测定泪液中视黄醇的来源 催泪剂分泌物和萃取物的层析(HPLC) 腺体放射自显影及泪腺后系统放射自显影 用放射性标记的维甲酸治疗动物。 2)研究眼泪中的维生素A与眼睛健康的关系 通过比较系统性给药对角膜表面的标记 正常动物和泪液受损动物体内放射性标记的维甲酸 通过尝试逆转动物角膜的角质化来发挥作用 因局部应用维甲酸导致泪液功能受损，以及 高效液相色谱法测定人和动物泪液中视黄醇浓度 眼表疾病。 3)研究维生素A在角膜中的作用机制，通过研究 维甲酸对培养和体内角膜细胞生长的影响 并通过对角膜中维甲酸代谢的研究，对其进行了高效液相分析 外用维甲酸对内皮细胞伤口愈合的影响。

The overall objective of the proposed work is to study the nutritive relationship of the tears to the cells of the ocular surface. It has recently been reported that retinol is present in tears. Since vitamin A may affect and be involved in the regulation and maturation of cells in the cornea and conjunctiva, the studies will initially focus on delivery of vitamin A to the ocular surface by the tears based on the hypothesis that ocular surface disease, especially keratinization, in patients with tear film abnormalities may in part be due to inadequate delivery of vitamin A to the ocular surface epithelium by the tears. The specific aims of the project are to: 1) determine the source of retinol in the tears by high pressure liquid chromatography (HPLC) of secretions and extracts of the tear-producing glands and by radioautography of tear-producing glands after system treatment of animals with radiolabeled retinoids. 2) study the relationship of vitamin A in tears to the health of the ocular surface by comparing labeling of the cornea by systematically administered radiolabeled retinoids in normal animals and animals with impaired tear function, by attempting to reverse keratinization of corneas in animals with impaired tear function by topical application of retinoids, and by measuring retinol concentration by HPLC in tears of humans and animals with ocular surface disease. 3) study the mechanisms of action of vitamin A in the cornea, by studies of the effect of retinoids on growth of corneal cells in culture and in vivo, and by HPLC analysis of retinoid metabolism in the cornea by studies of the effect of topical retinoids on endothelial wound healing.