VOLUME REGULATION IN NORMAL AND CATARACTOUS LENSES

任何组织的电学性质都与 维持细胞体积。该透镜的电学特性是 由四个解剖结构赋予的：前膜 上皮，晶状体纤维的膜，缝隙连接电子 连接晶状体的细胞和微小的细胞间体积。我们正在使用 微电极用于研究人工晶状体的频域阻抗 正常情况下和受到异常离子沐浴介质的影响 合成的或含有药理成分的。阻抗研究使我们能够 分别确定描述每个解剖结构的电参数 结构，从而隔离每个实验的效果 对正确结构的干预。通过这种方式，我们希望将一种 了解正常晶状体和细胞体积是如何维持的。同样的研究 然后在白内障的实验模型上进行实验，我们希望 找出可能导致在这些方面失去数量调节的早期变化 隐形眼镜。

The electrical properties of any tissue are intimately related to the maintenance of cellular volume. The electrical properties of the lens are conferred by four anatomical structures: the membranes of the anterior epithelium, the membranes of the lens' fibers, the gap junctions electrically connecting the lens' cells, and the tiny intercellular volume. We are using microelectrodes to perform frequency domain impedance studies of the lens under normal conditions and when subjected to bathing media of abnormal ionic composition or containing pharmacological agents. Impedance studies allow us to separately determine the electrical parameters describing each anatomical structure within the lens, thereby isolating the effect of each experimental intervention to the correct structure. In this way we hope to characterize a normal lens and understand how cellular volume is maintained. The same studies will then be performed on experimental models of cataracts and we hope to identify early changes that might lead to a loss of volume regulation in these lenses.