ANALYSIS AND CONTROL OF CHROMOSOME MOVEMENT

同一染色体在生命和电子显微镜中的相关性研究 都被提出了。第一个目标是在了解染色体方面取得进展。 分发。我们将研究染色体与染色体的初始相互作用 纺锤体，寻找动粒微管的起源及其 与主轴的其余部分整合。第二，我们会调查 染色体显微操作后的纺锤体结构。其目标是创造 与单个染色体相关的微管是 易于识别，其中微管之间的机械连接 直接暴露了。 有丝分裂力量和纺锤体机械性能的定量研究 也提出了。我们将使用经过校准的玻璃纤维来产生已知的力 当尖端偏转给定的范围时。我们会尝试确定 主轴产生的最大力，微管对 弯曲，以及染色体速度和抗性之间的关系 有动静。

Correlated studies of the same chromosome in life and in the electron microscope are proposed. The first aim is progress toward understanding chromosome distribution. We would examine the initial interactions of chromosomes with the spindle, seeking information on the origin of kinetochore microtubules and their integration with those of the rest of the spindle. Second, we would investigate spindle structure after chromosome micromanipulation. The aim is to create situations in which the microtubules associated with a single chromosome are readily identifiable and in which mechanical linkage between microtubules is directly revealed. Quantitative studies of mitotic forces and spindle mechanical properties are also proposed. We would use glass fibers calibrated to produce a known force when the tip is deflected by a given extent. We would attempt to determine the maximum force produced by the spindle, the resistance of microtubules to bending, and the relationship between chromosome velocity and the resistance to movement.