OPERON CONTROL BY INTERACTING REGULATORY ELEMENTS

了解基因表达是如何控制的，这是 发现细胞正常和异常生长的机制 以及在所有生物系统中的分化。最近的信息表明 大量积极和消极的监管因素是 用于控制细菌中的个体基因活性以及 在多细胞生物体中。越来越明显的是， 这些监管组成部分之间的相互作用，而不是它们各自的 活动，是基因功能的关键。考虑到这一点，我们希望 继续我们对控制ILVBN的因素的研究和 大肠杆菌ompB操纵子。这项工作的目标是双重的： 第一，要更加透彻地了解融合之间的相互作用 宿主因子(IHF)和ilvBN衰减系统。这项工作将涉及 转录抗终止蛋白的体内外分析 IHF在ilvBN导联的活性。第二个目标是理解 多种相互作用的DNA结合蛋白在ompB表达中的作用 与ompB启动子区域连接。生物化学和遗传学相结合的方法 将被用来试图确定生理意义和 这些相互作用所涉及的确切分子机制 参与ompB转录调控的蛋白质。

The understanding of how gene expression is controlled is fundamental to discovering the mechanisms involved in normal and abnormal cellular growth and differentiation in all biological systems. Recent information suggests that a large number of positive and negative regulatory factors are operative in controlling individual gene activity in bacteria as well as in multicellular organisms. it is becoming increasingly clear that the interplay of these regulatory components, rather than their individual activities, is critical to gene function. With this in mind we wish to continue our study of the factors involved in the control of the ilvBN and ompB operons of Escherichia coli. The goals of this work are twofold: First, to more thoroughly understand the interaction between integration host factor (IHF) and the ilvBN attenuation system. This work will involve in vivo and in vitro analysis of the transcriptional antitermination activity of IHF in the ilvBN leader. The second goal is to understand the role in ompB expression of a number of DNA binding proteins that interact with the ompB promoter region. A combined biochemical and genetic approach will be used in an attempt to determine the physiological significance and the precise molecular mechanisms involved in the interplay of these proteins in the regulation of ompB transcription.