VIROLOGIC & IMMUNOLOGIC STUDIES OF MURINE CMV RETINITIS

描述：（改编自申请人摘要）申请人已 最近证明，将MCMV接种到睫状体上间隙， 免疫活性BALB/c小鼠的视网膜炎， 在免疫功能低下的人眼中观察到相同的组织病理学特征 CMV视网膜炎患者。 第一个拟议的实验将是 在免疫活性BALB/c小鼠中进行。 研究者将使用 病毒学、免疫学和分子生物学技术， 接种病毒后病毒复制的程度和位置 进入睫状体上间隙、病毒潜伏部位和全身 免疫反应 这些实验之后将进行 剂量反应实验，以选择导致最小 在最初感染时视网膜受累， 视网膜（和/或其他眼部结构）中的潜伏期。 申请人 然后建议使用各种特异性（T细胞亚群的耗竭）， 非特异性免疫抑制策略以重新激活病毒， 产生视网膜炎。

DESCRIPTION: (Adapted from applicant's abstract) The applicant has recently demonstrated that inoculation of MCMV into the supraciliary space of immunocompetent BALB/c mice results in a retinitis that has some of the same histopathologic features observed in eyes of immunocompromised human patients with CMV retinitis. The first of the proposed experiments will be performed in immunocompetent BALB/c mice. The investigator will use virologic, immunologic and molecular biological techniques to determine the extent and location of virus replication following inoculation of virus into the supraciliary space, sites of viral latency, and the systemic immune response. These experiments will be followed by performance of dose-response experiments to select a dose of virus that results in minimal retinal involvement at the time of initial infection but which produces latency in the retina (and/or in other ocular structures). The applicant then proposes to use various specific (depletion of T cell subsets) and non-specific strategies of immunosuppression to reactivate virus and produce retinitis.