OPERON CONTROL BY INTERACTING REGULATORY ELEMENTS

了解基因表达是如何被控制的， 发现正常和异常细胞生长的机制 和分化。 最近的信息显示 大量的积极和消极的调节因素 控制细菌中的单个基因活性， 在多细胞生物中。越来越清楚的是， 这些监管组件的相互作用，而不是它们各自的 对基因功能至关重要。 考虑到这一点，我们希望 继续研究控制ilvBN的因素， 大肠杆菌ompB操纵子 这项工作有两个目标： 第一，要更加深入地理解整合之间的相互作用 宿主因子（IHF）和ilvBN衰减系统。 这项工作将涉及 转录抗终止的体内和体外分析 IHF在ilvBN领导人中的活动。 第二个目标是了解 在ompB表达中起作用的许多DNA结合蛋白， 与ompB启动子区连接。 生物化学和遗传学的结合 将被用来试图确定生理意义， 这些分子之间相互作用的精确分子机制 蛋白质在ompB转录调控。

The understanding of how gene expression is controlled is fundamental to discovering the mechanisms involved in normal and abnormal cellular growth and differentiation in all biological systems. Recent information suggests that a large number of positive and negative regulatory factors are operative in controlling individual gene activity in bacteria as well as in multicellular organisms. it is becoming increasingly clear that the interplay of these regulatory components, rather than their individual activities, is critical to gene function. With this in mind we wish to continue our study of the factors involved in the control of the ilvBN and ompB operons of Escherichia coli. The goals of this work are twofold: First, to more thoroughly understand the interaction between integration host factor (IHF) and the ilvBN attenuation system. This work will involve in vivo and in vitro analysis of the transcriptional antitermination activity of IHF in the ilvBN leader. The second goal is to understand the role in ompB expression of a number of DNA binding proteins that interact with the ompB promoter region. A combined biochemical and genetic approach will be used in an attempt to determine the physiological significance and the precise molecular mechanisms involved in the interplay of these proteins in the regulation of ompB transcription.