Background-free imaging of single membrane-receptors with nanophosphors

该项目旨在开发纳米磷光信标和实时、超高灵敏度的功能成像，以提供大脑的图像。时间门控检测显微镜将使这些纳米荧光粉具有优异的光学对比度。纳米磷光体的抗体靶向将在其完整的生物学背景下对单个AMPA膜受体进行成像，这对于理解神经元信号至关重要。同时对神经元中的受体运输和活动进行成像将有助于揭示大脑动态活动的细节。这项技术有望帮助理解大脑的内部运作，并提供对其功能的洞察。

This project aims to develop nanophosphor beacons and real-time, ultrahigh-sensitivity functional imaging to provide a picture of the brain. Time-gated detection microscopy will give these nanophosphors a superior optical contrast. The nanophosphors’ antibody-targeting will image single AMPA membrane receptors in their full biological context, crucial to understanding neuronal signalling. Simultaneous imaging of receptor trafficking and activity in neurons will help to uncover details of the dynamic activity in the brain. This technology is expected to help understand the inner workings of the brain and provide insights into its functioning.