STRUCTURE AND FUNCTION OF HEART MEMBRANE PROTEINS

本研究的总体目标是阐明 电子转移与质子传能机制 线粒体细胞色素bc 1复合物的易位 呼吸链 在即将到来的项目期间， 将集中在确定质子易位反应和 参与这些反应的氧化还原蛋白。 实验的具体目的是定位泛醌和/或 泛醇结合位点，推测在bc 1的铁硫蛋白上 复合物，其中质子与电子同时释放 转移 将进行相关研究，以测试类似的 细胞色素B上的醌/醌醇结合位点。 第二个目标 实验将测试细胞色素B是否是跨膜的， 氧化还原连接质子泵。 这些研究将用线粒体和两种 需氧细菌，它提供了独特的优势，以回答方面的问题， 这些问题不容易通过实验来回答， 线粒体 所用的方法包括纯化膜 呼吸链复合物进入磷脂囊泡， 标签。

The overall objective of this research is to elucidate the pathway of electron transfer and the mechanism of energy transducing proton translocation in the cytochrome bc1 complex of the mitochondrial respiratory chain. During the forth-coming project period experimentation will be focused on identifying the proton translocation reactions and the redox proteins involved in those reactions. The specific aims of the experimentation are to locate a ubiquinone and/or ubiquinol binding site, presumably on the iron-sulfur protein of the bc1 complex, from which protons are released coincident with electron transfer. Related studies will be conducted to test for a similar quinone/quinol binding site on cytochrome b. A second aim of the experimentation will be to test whether cytochrome b is a transmembranous, redox linked proton pump. These studies will be carried out with mitochondria and with two species of aerobic bacteria which offer unique advantages to answering aspects of these questions which are not as readily answered by experimentation with mitochondria. The methods to be used include purification of membranous respiratory chain complexes into phospholipid vesicles, and photoaffinity labeling.