CONTROL OF VIRUS INDUCED LYSIS

许多病毒诱导精确的预定细胞裂解来终止 感染循环。这种裂解的机制及其时间调节 将被研究，最初在噬菌体Lambda和phiX174中。 Lambdas和phiX174 E的主要裂解蛋白 基因，将被提纯。针对这些蛋白质产生的抗血清将是 用于研究这些蛋白质在体内的新陈代谢。的影响 这些蛋白质对重组膜小泡通透性的影响 要下定决心。抵抗这些蛋白质作用的宿主突变体将 被分析以确定细胞中的哪些结构被附着，或 抵抗，这是一种溶解机制。病毒裂解基因的突变将是 通过DNA测序建立裂解过程中的功能结构域 蛋白质一级结构。S和E的杂交融合基因将是 在体外构建，以确定裂解特异性可能在哪里 控制住了。这些方法有望解决预定的溶解 这一现象在很大程度上，并有助于对 蛋白质的膜运输。此外，一种自裂解菌株 大肠埃希氏菌将被构建为克隆载体。 最后，对裂解机制的研究将扩展到动物病毒。 流感病毒裂解突变体将被分离并鉴定 开发一种治疗方案的长期目标是 在被感染的细胞中诱导不适当的裂解，从而感染 缩减了。

Many viruses induce precisely scheduled cell lysis to terminate the infective cycle. The mechanism of this lysis and its temporal regulation will be investigated, initially in the bacteriophages Lambda and PhiX174. The principle lysis proteins, the products of the LambdaS and PhiX174 E genes, will be purified. Antisera raised against these proteins will be used to study the metabolism of these proteins in vivo. The effect of these proteins on the permeability of reconstituted membrane vesicles will be determined. Host mutants resistant to the action of these proteins will be analyzed to determine what structures in the cell are attached by, or resist, the lysis mechanism. Mutations in the viral lysis genes will be analyzed by DNA sequencing to establish functional domains in the lysis protein primary structure. Hybrid fusion genes between S and E will be constructed in vitro, to determine where lysis specificity may be controlled. These methods are expected to resolve the scheduled lysis phenomenon substantially, and to contribute to the general understanding of the membrane transport of proteins. In addition, a self-lysing strain of Escherischia coli will be constructed for use as a cloning vehicle. Finally, the study of lysis mechanisms will be extended to animal viruses. Lysis mutants of influenza virus will be isolated and characterized with the long range goal of developing a therapeutic regimen in which inappropriate lysis is induced in infected cells and thus infection curtailed.