ANESTHETIC ACTION AND VIRUS REPLICATION

拟议的研究将检查挥发性物质的作用。 吸入麻醉药对麻疹病毒生物化学的影响 病毒感染患者的体外和致病机制 正在接受手术和麻醉。麻疹病毒将在 组织培养和暴露于各种挥发性气体吸入 麻醉剂。氟烷、安氟醚或 异氟醚影响病毒大分子合成和成熟， 特别是蛋白质的合成和加工，将被研究 与细胞内蛋白质的合成和加工相比 未受感染的培养物暴露于和不暴露于挥发性 麻醉剂。我们将确定麻醉剂是否会诱导 蛋白质合成的抑制发生在转录水平 和/或翻译级别。麻醉剂引起的精神障碍 病毒蛋白的磷酸化和糖基化将被 检查过了。麻醉诱导的细胞水平的变化 介体，如那些控制磷酸化和 糖基化将被评估，并与观察到的变化相关联 在蛋白质加工方面。相信这些研究将有助于 阐明麻醉剂在诱导改变中所起的作用 在蛋白质合成和加工中通过改变细胞内 病毒复制过程中的信使水平和/或转导。 迄今为止在儿科人群中进行的临床研究有 提出了一个重要的临床和经济方向 麻醉的实践。本提案中所述的研究 将在成年人群中继续并扩大这些实验 患有上呼吸道感染并正在接受 妇科手术的麻醉剂。通过在体内将这些联系起来 结果根据我们的体外结果，我们希望建立一家公司 我们临床发现的分子基础。

The proposed research will examine the action of volatile inhalational anesthetics on the biochemistry of measles virus in vitro and on the pathogenesis of virus infections in patients undergoing surgery and anesthesia. Measles virus will be grown in tissue culture and exposed to various volatile inhalational anesthetics. The mechanism by which halothane, enflurane, or isoflurane affect viral macromolecular synthesis and maturation, especially protein synthesis and processing, will be investigated and compared to cellular protein synthesis and processing in uninfected cultures exposed and not exposed to the volatile anesthetics. We will determine whether anesthetic induced inhibition of protein synthesis occurs at the transcriptional and/or translational level. Anesthetic induced perturbations in phosphorylation and glycosylation of virus proteins will be examined. Anesthetic induced alterations in the levels of cellular mediators such as those which control phosphorylation and glycosylation will be assessed and correlated to observed changes in protein processing. It is believed these studies will help to elucidate the role that anesthetics play in inducing alterations in protein synthesis and processing by altering intracellular messenger levels and/or transduction during virus replication. The clinical studies to date in a pediatric population have suggested an important clinical and economical direction in the practice of anesthesia. The studies described in this proposal will continue and extend these experiments in an adult population who have an upper respiratory tract infection and are undergoing anesthesia for gynecological surgery. By correlating these in vivo results with our in vitro results we hope to establish a firm molecular basis for our clinical findings.