MUTATIONS AFFECTING THE PRODUCTION OF MATURE RNAS

因此，研究其调控、合成和表达， 真核生物的tRNA可以在酵母中完成，酵母是一种简单的真核生物， 可以进行组合的遗传和生物化学研究。 研究 是针对分离突变体或建立条件， 合成或表达异常。 实现这一点的两种方法是 描述了：（1）分离突变体，其条件性地丧失了以下能力： 抑制无义突变，也不能在非允许的 温度（导致细胞失去抑制基因的突变类型） 将通过重建携带突变的抑制菌株来研究活性 在影响酵母细胞生长的基因中）;（2）氚 用功能性RNA聚合酶III自杀细胞。 变种人将会 进行遗传分析以确定互补组的数量， 确定突变是否影响一种以上的tRNA。 的RNA 将进行生物化学分析，以建立 处理事件，以确定修饰的碱基在tRNA功能中的作用，以及 研究RNA聚合酶III的亚基。

It is proposed that a study of the regulation, synthesis, and expression of eukaryotic tRNA can be accomplished in yeast - a simple eukaryote in which combined genetic and biochemical investigations can be conducted. The research is directed towards isolating mutants or establishing conditions for which tRNA synthesis or expression is aberrant. Two approaches to accomplishing this are described: (1) isolation of mutants which conditionally lose the ability to suppress nonsense mutations and also fail to grow at the nonpermissive temperature (the types of mutations which cause cells to lose suppressor activity will be studied by reconstructing suppressor stains bearing mutations in genes affecting well described aspects of yeast cells growth); (2) tritium suicide of cells with functional RNA polymerase III. The mutants will be analyzed genetically to determine the number of complementation groups and to determine whether the mutations affect more than one species of tRNA. The RNA from the cells will be analyzed biochemically to establish a pathway of processing events, to determine the role of modified bases in tRNA function, and to study the subunits of RNA polymerase III.