NMR ANALYSIS OF RNA STRUCTURE AND DYNAMICS

RNA寡聚体的构象和动力学性质将是 考察 具体而言，将详细研究的序列是： (1)三个双螺旋形成寡聚体，其中一个同源DNA 一个序列已经被研究过了，它可能表现出不同的结构 温度和类似的控制顺序不应改变， (ii)R17病毒RNA外壳蛋白结合位点的变体 对外壳蛋白表现出不同的亲和力。 述蛋白质是 参与R17编码的复制酶基因的翻译抑制。 这些序列将用作完全碱基配对的RNA的模型， 发夹/凸起环是折叠RNA结构的常见元件， 发生在自然界。 不寻常的结构特征将充分相关， 它在生物功能中的作用。 主要工具是核磁共振，该项目旨在 开发和完善质子-质子核的应用。 Overhauser 增强和碳-13光谱。 前者将是解决方案， 后者将产生关于RNA内部动力学的信息。 是 重要的是要把重点放在RNA结构，这是关键的生物分子， DNA编码的信息的表达。 多年来，这个项目 帮助创造了RNA寡聚体合成的突破， 用于分配和评估它们的复杂NMR谱。 这项工作也 随着其他实验室的进展，现在可以确定三个- 在这个复杂的系统中。 该项目将大大有助于令人兴奋的进展， 在从溶液核磁共振产生生物分子的详细模型中， 数据 它还将有助于开发和应用新的方法， 对基因表达控制的理解不同。

The conformational and dynamical properties of RNA oligomers will be examined. Specifically, the sequences that will be studied in detail are: (1) three double helix forming oligomers, one for which the cognate DNA sequence has already been studied, one that may exhibit varying structure with temperature and a similar control sequence that should not vary, and (ii) variants of the binding site for the coat protein of R17 viral RNA that display differential affinities for the coat protein. The protein is involved in the translational repression of the R17-encoded replicase gene. These sequences will be used as models for fully base-paired RNA and hairpin/bulge loops that are common elements of folded RNA structures that occur in nature. unusual structural features will be related as fully as possible to their role in biological function. The principle tool is nuclear magnetic resonance and the project seeks to develop and refine applications of proton-proton nuclear. Overhauser enhancements and carbon-13 spectra. The former will be solution, and the latter will yield information on the internal dynamics of RNA. It is important to focus on RNA structures which are critical biomolecules in the expression of information coded in DNA. Over the years this project has helped create breakthrough in the synthesis of RNA oligomers and methods for assigning and evaluating their complex nmr spectra. This work as well as progress in other labs now make it feasible to determine three- dimensional structures in this difficult system. This project will contribute substantially to the exciting progress being made in the generation of detailed models of biomolecules from solution nmr data. It will also help develop and apply new methods where they can made a difference in the understanding of the control of genetic expression.