ION MOBILITY SPECTROMETRY

用气体分析复杂混合物，如生物液体 色谱法通常需要使用选择性检测器 歧视干扰物种和/或增强对 所需的化合物。 然而，除了质谱仪之外，大多数 GC检测器在宽范围内难以或不可能操作。 多种选择性和非选择性模式。 虽然不是为了 提供质谱仪提供的数据的范围和范围，离子 迁移率谱仪能够定量和低分辨率 有机化合物的定性检测。 当与普通 采用选择性检测器，如电子捕获和 氮磷探测器，离子迁移谱仪提供了 可调选择性的独特优势。 由于其多功能性和 与质谱仪相比，该仪器成本低， 在生物样品中常规筛选许多药物的巨大潜力。 在过去的资助期间，我们扩大了离子的能力， 通过发展傅里叶变换模式的迁移率光谱法 的操作。 傅里叶变换离子迁移谱与 在本申请中提出的超临界流体色谱将 生产一种强大的分析仪器， 生物学上重要的微量元素的定性测定 挥发性不足以被气体洗脱的化合物 色谱分离，但包含在混合物中太复杂，无法通过色谱分离。 液相色谱法

The analysis of complex mixtures such as biological fluids by gas chromatography often requires the use of selective detectors to discriminate against interfering species and/or enhance the response to desired compounds. However, with the exception of mass spectrometers, most GC detectors are either difficult or impossible to operate in a wide variety of selective and nonselective modes. Although not designed to provide the range and scope of data provided by mass spectrometers, the ion mobility spectrometer is capable of quantitative and low resolution qualitative detection of organic compounds. When compared with commonly employed selective detectors such as the electron capture and nitrogen-phosphorus detectors, the ion mobility spectrometer offers the distinct advantage of tunable selectivity. Because of its versatility and low cost when compared to that of mass spectrometers, the instrument has great potential for routine screening of many drugs in biological samples. During the past funding period we have expanded the capabilities of ion mobility spectrometry through the development of a Fourier transform mode of operation. Coupling Fourier transform ion mobility spectrometry with supercritical fluid chromatography as is proposed in this application will produce a powerful analytical instrument for both quantitative and qualitative determination of trace quantities of biologically important compounds that are not sufficiently volatile to be eluted by gas chromatography but are contained in mixtures too complex to be separated by liquid chromatography.