REGULATION OF YEAST RIBOSOMAL PROTEIN GENES

核糖体蛋白的合成在酵母中受到协调调节。我们 最近分离出了一些编码核糖体蛋白的基因 并证明了协调调节可以发生在 转录或翻译的水平，取决于细胞 条件。我计划用这些克隆的基因来研究这种机制 协调控制。其次，我希望利用这些基因和它们的 转录本作为研究信使核糖核酸加工和基因复制的模型。三 将使用各种方法。1.我已经分离出一个可能的调控突变体 与核糖体蛋白L3的基因tcm1紧密连锁。我计划这样做 分离更多这样的突变体并确定它们对坐标的影响 监管。2.大多数核糖体蛋白基因转录时都很大 含有内含子的前体。有多个温度 阻止这些mRNA加工的敏感突变体 (rna 2-rna 11)。我已经分离出一种抑制TS的抑制子 其中五个RNA突变的表型。我计划调查核糖核酸 加工过程中生化与遗传的关系分析 在这些加工缺陷突变体和它们的抑制子之间。3.的 克隆了近20个核糖体基因，只有两个紧密连锁；此外， 这两个相连的基因在基因组的另一个位置复制。我 计划对这两组相连的基因进行测序并分析它们的功能 通过体外诱变来了解基因的作用机制 复制及其维持复制核糖体蛋白的必要性 基因组中的基因。

Synthesis of ribosomal proteins is coordinately regulated in yeast. We have recently isolated a number of genes which code for ribosomal proteins and have demonstrated that the coordinate regulation can occur, either at the level of transcription or translation, depending on cellular conditions. I plan to use these cloned genes to investigate the mechanism of coordinate control. Secondly, I wish to utilize these genes and their transcripts as models to study mRNA processing and gene duplication. Three approaches will be used. 1. I have isolated a putative regulatory mutant closely linked to tcm1, the gene for ribosomal protein L3. I plan to isolate more such mutants and determine their effect on coordinate regulation. 2. Most ribosomal protein genes are transcribed as large precursors which contain introns. There are a number of temperature sensitive mutants which prevent the processing of these mRNAs (rna2-rna11). I have isolated a suppressor which suppresses the ts phenotype of five of these rna mutations. I plan to investigate RNA processing by a biochemical and genetic analysis of the relationship between these procesing defective mutants and their suppressors. 3. Of nearly 20 ribosomal genes cloned, only two are closely linked; furthermore, these two linked genes are duplicated at one other site in the genome. I plan to sequence these two sets of linked genes and analyze their function by in vitro mutagenesis to gain some insight into the mechanism of gene duplication and the necessity for maintaining duplicate ribosomal protein genes in the genome.