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STRUCTURE AND FUNCTION OF THE NUCLEAR ENVELOPE

STRUCTURE AND FUNCTION OF THE NUCLEAR ENVELOPE
核膜的结构和功能
批准号：
3281935
负责人：
JOHN W SEDAT
金额：
$9.66万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
1983
资助国家：
美国
项目状态：
已结题
起止时间：
1983-12-01 至 1986-11-30
来源：
https://reporter.nih.gov/project-details/3281935
关键词：
affinity chromatography chemical structure function early embryonic stage electron microscopy gel electrophoresis immunological substance monoclonal antibody nonmammalian vertebrate embryology nuclear membrane oogenesis surface antigens

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中文摘要
翻译
该建议建立在已建立的单克隆抗体库的基础上， 果蝇核蛋白和最近开发的研究工具 亚细胞结构的三维重建。 这项建议的重点是一个子集的抗体标记的核 信封. 使用电子显微镜， 将建立核包层。 进一步的相关性 核膜亚结构的间期染色体的 将完成三维图像处理技术。 抗原将被纯化并通过生物化学方法进行广泛表征。 程序.
英文摘要
This proposal builds on the established library of monoclonal antibodies to Drosophila nuclear protein and the recently developed tools of three-dimensional reconstruction of subcellular structures. This proposal focusses on a subset of the antibodies that label the nuclear envelope. Using electron microscopy, the substructure and location within the nuclear envelope will be established. Further correlation of the nuclear envelope substructures to the interphase chromosomes by the three-dimensional image processing techniques will be accomplished. The antigen will be purified and extensively characterized by biochemical procedures.
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