WILD-DERIVED GENETIC VARIATION

这是一个计划，旨在进行一系列协调一致的项目 哺乳动物基因组的调节和结构。我们建议继续 识别和表征被确认为 在完整小鼠中的多态，并检查两者之间的关系 在蛋白质、mRNA和DNA水平上研究基因表达的变化 序列差异。主要的焦点是对遗传学和 一组雄激素反应基因的分子生物学，包括 β-葡萄糖醛酸苷酶，肾脏中其他雄激素诱导序列，以及 颌下腺肾素。第二个项目涉及审查 出现在不同转录本中的重复DNA序列 基因产品家族。该计划的第三个领域是研究 小鼠急性时相反应的遗传学和分子生物学研究 作为这种反应一部分的产物的基因特征。 该程序的一个附加元素包含CELL的一个强大组件 生物学在亚细胞遗传和生化表征中的应用 β-葡萄糖醛酸苷酶的定位。这项工作得到了一种基因的补充 质膜蛋白结构域的分析。这项工作得到了 开发包括新的同源基因在内的一系列小鼠遗传资源 品系、野生派生种群和广泛的育种计划。

This is a program to carry on a coordinated series of projects on the regulation and structure of the mammalian genome. We propose to continue to identify and characterize regulatory variants recognized as polymorphisms in intact mice, and to examine the relationship between altered gene expression studied at the protein and mRNA level and DNA sequence differences. The major focus is a study of the genetics and molecular biology of a set of androgen responsive genes, including Beta-glucuronidase, other androgen-inducible sequences in the kidney, and submaxillary gland renin. A second project concerns the examination of repetitive DNA sequences which appear within transcripts in various families of gene products. A third area in the program is the study of the genetics and molecular biology of the acute phase response in mice and the genetic characterization of the products which are part of this response. An additional element of this program contains a strong component of cell biology in the genetic and biochemical characterization of the subcellular localization of Beta-glucuronidase. This work is complemented by a genetic analysis of plasma membrane protein domains. The work is supported by the development of a series of mouse genetic resources, including new congenic strains, wild-derived stocks, and an extensive breeding program.