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NMR STUDIES OF METABOLIC PATHWAYS IN VIVO AND IN VITRO

NMR STUDIES OF METABOLIC PATHWAYS IN VIVO AND IN VITRO
体内和体外代谢途径的 NMR 研究
批准号：
3281597
负责人：
ALASTAIR I SCOTT
金额：
$21.63万
依托单位：
TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
依托单位国家：
美国
项目类别：
财政年份：
1983
资助国家：
美国
项目状态：
已结题
起止时间：
1983-03-01 至 1988-02-29
来源：
https://reporter.nih.gov/project-details/3281597
关键词：
Fungi Krebs' cycle bacteria biotransformation cell free system cephalosporins chemical reaction chemical synthesis cholesterol chymotrypsin clinical biomedical equipment cryoscience deuterium eicosanoid metabolism erythrocytes fatty acid biosynthesis fatty acid synthase fermentation fibroblasts gluconeogenesis glycolysis human tissue luciferin monooxygenase metalloenzyme methionine methylmalonyl coA epimerase neoplastic cell noninvasive diagnosis nuclear magnetic resonance spectroscopy nutrition related tag o aminobenzoate pancreatic ribonuclease papain penicillins plants prostaglandin endoperoxide synthase purine /pyrimidine metabolism radiopharmacology squalene stable isotope diagnosis steroid biosynthesis tetracyclines tissue /cell culture trypsin vitamin B12 compound vitamin biosynthesis

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中文摘要
翻译
使用大口径、高场(300-500 MHz)、 高分辨率核磁共振波谱在生物代谢研究中的应用 将进行系统和体外实验。乘法标号的使用 通过区域特异性合成制备的底物(13C、15N、2H)将是 将化学位移分配给未知和短命物种的价值。它 建议使用低温酶学和流动细胞来研究 生化反应中的碳、氢和氮，以识别 迄今未被检测到的物种，并设计非侵入性方法来 微克量的生物重要分子的分析 哺乳动物、细菌和植物细胞的代谢，以及整个 灌流的器官和昆虫。
英文摘要
Development of techniques using wide-bore, high-field (300-500 MHz), high-resolution NMR spectroscopy to study metabolic processes in living systems and in vitro will be undertaken. The use of multiply labeled substrates (13C, 15N, 2H) prepared by regiospecific synthesis will be of value in assigning chemical shifts to unknown and short-lived species. It is proposed to use cryoenzymology and flow cells to study the flux of carbon, hydrogen and nitrogen in biochemical reactions, to identify hitherto undetected species and to devise non-invasive methods for the analysis of microgram quantities of biologically important molecules during metabolism in mammalian, bacterial and plant cells, as well as whole, perfused organs and insects.
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