INTERPHASE SOLUBILITY AND CHRMOATOGRAPHIC RETENTION

拟议的工作将是一项实验和理论工作相结合的工作 两个小组，旨在为预测分子 物质的“相间”中的溶解度，特别是静止的 杠杆相液相色谱(RPLC)的碳氢化合物相 包括两亲性聚集体，例如脂质双层膜，囊泡， 和胶束。 我们之前已经发展了统计力学理论来预测 膜和胶束中的分子组织。预测是正确的。 与小角中子x射线衍射结果一致 散射、~2H、~(13)C、~(19)F核磁共振和状态方程测量。这个 主要结论是链分子中间相不是主体相 物质，但与界面相极为相似。体相 综合性质和分配系数的热力学是 不适用于这些系统；在这里，我们的目标是开发更合适的 溶解度的热力学和统计力学理论 InterPhase。 我们的实验工作一直致力于理论和实践 色谱系统的应用表征。在这里，我们的目标是测试 通过对溶质大小和尺寸的系统研究，建立了严格的理论模型 接枝产物的化学性质、链长和表面密度 相间链，并通过建立有效的参考状态。 这项工作应直接关系到：i)RPLC的保留机制， 应用最广、发展最快的层析方法 分离，以及II)类化合物的基本物理化学 脂双层、囊泡和生物膜的性质，并且可以 为了解麻醉剂的分子机制提供了基础 毒品行动。

The proposed work, to be a combined experimental and theoretical effort of two groups, aims to provide a foundation for predicting molecular solubilities within "interphases" of matter, especially the stationary hydrocarbon phases of leversed Phase Liquid Chromatography, (RPLC), and including amphiphilic aggregates such as lipid bilayer membranes, vesicles, and micelles. We have previously developed statistical mechanical theory to predict molecular organization in membranes and micelles. Predictions are in good agreement with results of x-ray diffraction, small angle neutron scattering, 2H, 13C, 19F NMR, and equation of state measurements. The principal conclusion is that chain molecule interphases are not bulk phases of matter, but bear close resemblance to interfacial phases. Bulk phase thermodynamics of colligative properties and of partition coefficients is not applicable to these systems; here we aim to develop more appropriate thermodynamic and statistical mechanical theory for solubilities in interphases. Our experimental efforts have been directed toward both theoretical and applied characterization of chromatographic systems. Here, we aim to test rigorously theoretical models through systematic study of solute size and chemical character, chain length and surface density of the grafted interphase chains, and through the establishment of valid reference states. This work should have direct bearing on: i) Retention mechanisms of RPLC, the most widely used and rapidly growing method of chromatographic separation, and ii) Fundamental physical chemistry of colligative properties of lipid bilayers, vesicle, and biological membranes, and may provide a foundation for understanding molecular mechanisms of anesthetic drug action.