MECHANISMS IN RESPIRATION

这项工作涉及蛋白质的一般特性 参与细菌呼吸。 这其中的具体问题 建议是细胞色素claa3和Rieske铁/硫蛋白 分离自极端嗜热需氧菌栖热菌 嗜热菌 工作的总体目标是提供 耦合质子迁移机制的物理学研究 和电子转移。 使用的方法包括一般技术 蛋白质纯化和表征;那些重组 DNA技术，用于分离和测序感兴趣的基因;以及 物理方法，包括EPR、ENDOR、穆斯堡尔和共振 拉曼光谱。 动力学研究是为 分子氧中间体的检测和表征 减少，并用于检测光谱扰动的 泡状细胞色素aa3在极化作用下 电场 基于这样的信念， 需要详细的三维结构， 我们正在努力使这些蛋白质结晶。

This work involves the general characterization of proteins involved in bacterial respiration. The specific subjects of this proposal are the cytochrome claa3 and Rieske iron/sulfur protein isolated from the extremely thermophilic aerobe, Thermus thermophilus. The overall objective of the work is to provide physical insight on the mechanism of coupled proton translocation and electron transfer. The methods used include general techniques of protein purification and characterization; those of recombinant DNA technology to isolate and sequence the genes of interest; and physical methods including EPR, ENDOR, Mossbauer, and resonance Raman spectroscopies. Kinetic studies are designed for the detection and characterization of intermediates in dioxygen reduction, and for the detection of spectral perturbations of vesicular cytochrome aa3 under the influence of a polarizing electric field. Guided by the belief that understanding mechanisms in respiration will require detailed three-dimensional structures, we are making a serious effort to crystallize these proteins.