DYNAMICS OF ACTIN FILAMENTS IN NERVE GROWTH
DYNAMICS OF ACTIN FILAMENTS IN NERVE GROWTH
批准号:
3287273
负责人:
JAMES R BAMBURG
金额:
$12.73万
依托单位国家:
美国
项目类别:
财政年份:
1984
资助国家:
美国
项目状态:
已结题
起止时间:
1984-12-01 至 1989-11-30
关键词:
adenosinetriphosphatase affinity chromatography autoradiography brain brain metabolism cell growth regulation cell population study circular dichroism conformation density gradient ultracentrifugation electrofocusing electron microscopy gel electrophoresis glia ions local anesthetics messenger RNA neuropharmacology neurotrophic factors stoichiometry tissue /cell culture ultraviolet spectrometry wheat
中文摘要
使用基于G-肌动蛋白抑制蛋白水解的能力的G-肌动蛋白测定法,
为了研究DNA酶的活性,我们从脑中分离出一种蛋白质,
促进溶液中F-肌动蛋白的解聚。 这种蛋白质现在将
进一步表征其分子量、等电点、氨基酸
本发明的组合物及其与单独的F-肌动蛋白和在存在下的F-肌动蛋白相互作用的能力,
相关蛋白质如原肌球蛋白。
已经开发了DNA酶抑制测定的修改,其允许
在F-肌动蛋白存在的条件下,G-肌动蛋白的测定,
两个肌动蛋白池之间发生交换。 本试验将应用于
同步化CHO细胞中G和F-肌动蛋白浓度的测量
细胞周期
英文摘要
Using an assay for G-actin based on its ability to inhibit the hydrolytic
activity of DNAase, we have isolated a protein from brain which actively
promotes the depolymerization of F-actin in solution. This protein will now be
further characterized as to its molecular weight, isoelectric point, amino acid
composition and its ability to interact with F-actin alone and in the presence
of associated proteins such as tropomyosin.
A modification of the DNAase inhibition assay has been developed which permits
the assay of G-actin in the presence of F-actin under conditions where no
exchange between the two actin pools occurs. This assay will be applied to the
measurement of G and F-actin concentrations in synchronized CHO cells during the
cell cycle.
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会议论文
Role of cofilin pathology in mouse models of cognitive impairment
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批准号:8664331
-
项目类别:
-
资助金额:$18.59万
-
财政年份:2013
-
负责人:JAMES R BAMBURG
-
依托单位:
Role of cofilin pathology in mouse models of cognitive impairment
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批准号:8486049
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项目类别:
-
资助金额:$22.3万
-
财政年份:2013
-
负责人:JAMES R BAMBURG
-
依托单位:
ISOLATION AND CHARACTERIZATION OF CYTOPLASMIC COFILIN-ACTIN RODS
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批准号:8171304
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项目类别:
-
资助金额:$0.08万
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财政年份:2010
-
负责人:JAMES R BAMBURG
-
依托单位:
Training in Synaptic Neurobiology
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批准号:6768597
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项目类别:
-
资助金额:$25.73万
-
财政年份:2002
-
负责人:JAMES R BAMBURG
-
依托单位:
Training in Synaptic Neurobiology
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批准号:6919974
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项目类别:
-
资助金额:$25.81万
-
财政年份:2002
-
负责人:JAMES R BAMBURG
-
依托单位:
Training in Synaptic Neurobiology
-
批准号:7096532
-
项目类别:
-
资助金额:$19.29万
-
财政年份:2002
-
负责人:JAMES R BAMBURG
-
依托单位:
Training in Synaptic Neurobiology
-
批准号:6604211
-
项目类别:
-
资助金额:$25.73万
-
财政年份:2002
-
负责人:JAMES R BAMBURG
-
依托单位:
Training in Synaptic Neurobiology
-
批准号:6452374
-
项目类别:
-
资助金额:$24.71万
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财政年份:2002
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF-Actin Rods in Neurodegenerative Diseases
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批准号:6617076
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项目类别:
-
资助金额:$1.49万
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财政年份:2001
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF-Actin Rods in Neurodegenerative Diseases
-
批准号:6685913
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项目类别:
-
资助金额:$24.11万
-
财政年份:2001
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF-Actin Rods in Neurodegenerative Diseases
-
批准号:6826802
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项目类别:
-
资助金额:$24.11万
-
财政年份:2001
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF/cofilin-actin rods in neurodegenerative diseases
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批准号:7413273
-
项目类别:
-
资助金额:$32.12万
-
财政年份:2001
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF-Actin Rods in Neurodegenerative Diseases
-
批准号:6620582
-
项目类别:
-
资助金额:$24.11万
-
财政年份:2001
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF/cofilin-actin rods in neurodegenerative diseases
-
批准号:7591023
-
项目类别:
-
资助金额:$32.12万
-
财政年份:2001
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF-Actin Rods in Neurodegenerative Diseases
-
批准号:6419165
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项目类别:
-
资助金额:$26.87万
-
财政年份:2001
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF/cofilin-actin rods in neurodegenerative diseases
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批准号:7145732
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项目类别:
-
资助金额:$31.82万
-
财政年份:2000
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF/cofilin-actin rods in neurodegenerative diseases
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批准号:7236176
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项目类别:
-
资助金额:$32.08万
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财政年份:2000
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负责人:JAMES R BAMBURG
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依托单位:
ADF/COFILIN IN EARLY VERTEBRATE DEVELOPMENT
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批准号:2023273
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项目类别:
-
资助金额:$17.07万
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财政年份:1997
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负责人:JAMES R BAMBURG
-
依托单位:
ADF/COFILIN IN EARLY VERTEBRATE DEVELOPMENT
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批准号:2900873
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项目类别:
-
资助金额:$20.64万
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财政年份:1997
-
负责人:JAMES R BAMBURG
-
依托单位:
ADF/COFILIN IN EARLY VERTEBRATE DEVELOPMENT
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批准号:2685100
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项目类别:
-
资助金额:$19.55万
-
财政年份:1997
-
负责人:JAMES R BAMBURG
-
依托单位:
海外基金