HORMONAL REGULATION OF ENDOMETRIAL DIFFERENTIATION

干细胞介导分化的概念解释了这一过程 的组织再生提出了一个模型的循环更新， 人类子宫内膜。 类固醇激素对子宫内膜功能的调节 的主要部分，将于 研究的初始阶段。 因此，细胞分布 雌激素敏感性标志物，如雌二醇和孕酮受体 和雌二醇-17 β和20 α-二氢-孕酮脱氢酶 活动，在腺体和基质，他们的变化，在这些 月经周期中的细胞群，子宫内膜的调节 功能分化，在体外，和时间序列的 将研究类固醇-受体相互作用。 几个预测 关于上皮细胞的生长特征的方案， 性类固醇介导的生化分化将在 器官和细胞培养系统。 调节这些类固醇的作用， 抑制剂（12-0-十四烷酰基佛波醇乙酸酯）和促进剂（类维生素A） 差异化将有助于验证拟议的某些部分， 阴谋 各种转录和翻译的抑制剂将被 用于间接证明类固醇介导的分化是 事实上是由于基因转录。 同时相关的 生化终点与良好建立的形态学 分化将有助于识别分化阶段。

The concept of stem cell mediated differentiation that explains the process of tissue regenerations is proposed as a model for the cyclic renewal of human endometrium. Regulation of endometrial function by steroid hormones which forms the major part of the proposed scheme will be examined in the initial phase of the study. Accordingly, the cellular distribution of hormone-sensitive markers, such as receptors for estradiol and progesterone and estradiol-17Beta and 20Alpha-dihydro-progesterone dehydrogenase activities, within the glands and stroma, their alterations within these cell populations during the menstrual cycle, modulation of endometrial functional differentiation, in vitro, and the temporal sequence of steroid-receptor interactions will investigated. Several predictions of the scheme in regard to growth characteristics of epithelial cells and biochemical differentiation mediated by sex steroids will be followed in organ and cell culture system. Modulation of these steroid effects with inhibitors (12-0-tetradeconoyl phorbol acetate) and promotors (retinoids) of differentiation will serve to validate certain parts of the proposed scheme. Various inhibitors of transcription and translation will be utilized to indirectly demonstrate that steroid-mediated differentiation is indeed due to gene transcription. Simultaneous correlation of the biochemical end-points with the well established morphologic differentiation will help to identify the differentiation stages.