REGULATION OF LH RECEPTORS AND PROGESTERONE SECRETION

黄体分泌孕酮对于正常的黄体是必不可少的。 怀孕了。因此，阻断这种荷尔蒙分泌的方法 潜在的避孕应用。此外，中国的生物异常现象 这一系统会导致一些夫妇不育。的第一步 黄体生成素刺激孕酮分泌是 荷尔蒙与其受体的相互作用。因此，实验是 建议进一步描述这种相互作用。第一，中美之间的差异 黄体细胞对人绒毛膜促性腺激素的生物学反应 (人绒毛膜促性腺激素)和黄体生成素(黄体生成素)，它们与同一受体相互作用， 将会被研究。两种激素的脉冲作用对孕酮的影响 灌流系统中黄体组织切片的分泌将是 检查以确定hCG是否诱导长期的类固醇生成反应，如 在其他组织中可见。由于已知hCG-LH受体复合体 停留在黄体细胞表面的时间是黄体细胞表面黄体激素受体的50倍 我们还将比较hCG-LH受体的侧向迁移率 黄体细胞膜上的复合体和黄体黄体激素受体复合体 激光漂白回收技术。第二，最近的身份证明和 两种类固醇生成黄体细胞的特性导致 实验的目的是：1)确定黄体细胞的类型 来自滤泡颗粒细胞和膜细胞；2)细胞的特征 类型(S)，在体内结合黄体生成素和前列腺素F(2α)；以及3)定义性质 导致黄体退化的细胞间相互作用 黄体周期黄体的生殖周期或维持 怀孕了。在这些研究中将使用体内和体外程序。 第三，由于黄体分泌孕酮似乎是 更多地依赖于黄体生成素受体的数量，而不是 血液中这种荷尔蒙的水平，损失的机制和 将使用单抗研究促黄体生成素受体的更新 抗黄体生成素受体和/或允许黄体生成素或人绒毛膜促性腺激素 与受体共价连接。最后，GTP结合的作用 腺苷酸环化酶激素激活中的蛋白质将用 GTP的光亲和类似物。这些研究的结果将提供 关于调控的复杂生物机制的新信息 黄体分泌黄体酮。

Progesterone secretion by the corpus luteum is essential for normal pregnancy. Thus, methods to interrupt secretion of this hormone have potential contraceptive applications. In addition, biological anomolies in this system cause infertility in some couples. The first step in stimulation of progesterone secretion by luteinizing hormone (LH) is the interaction of the hormone with its receptor. Therefore, experiments are proposed to characterize further this interaction. First, differences in the biological response of luteal cells to human chorionic gonadotropin (hCG) and luteinizing hormone (LH), which interact with the same receptor, will be studied. The effects of pulses of the two hormones on progesterone secretion by slices of luteal tissue in a perifusion system will be examined to determine if hCG induces a prolonged steroidogenic response, as seen in other tissues. Since it is known that hCG-LH receptor complexes remain on the surface of luteal cells 50 times longer than LH-LH receptor complexes we will also compare the lateral mobility of hCG-LH receptor complexes and LH-LH receptor complexes in luteal cell membranes using a laser photobleach recovery technique. Second, recent identification and characterization of two types of steroidogenic luteal cells lead to experiments designed to: 1) identify the luteal cell types which originate from follicular granulosal and thecal cells; 2) characterize the cell type(s) which bind LH, and PGF(2 alpha) in vivo; and 3) define the nature of the cell-to-cell interactions which results in luteal regression during the reproductive cycle or maintenance of the corpus luteum during pregnancy. In vivo and in vitro procedures will be used in these studies. Third, since secretion of progesterone by the corpus luteum appears to be more dependent upon the number of receptors present for LH than on the levels of this hormone in blood, the mechanisms involved in loss and renewal of receptors for LH will be studied using monoclonal antibodies against the LH receptor and/or methods which allow LH or hCG to be covalently linked to the receptor. Finally, the role of the GTP-binding protein in hormonal activation of adenylate cyclase will be studied using photoaffinity analogs of GTP. The results of these studies will provide new information regarding the complex biological mechanisms which regulate progesterone secretion from the corpus luteum.