HORMONAL CONTROL OF DELAYED IMPLANTATION

要检验的主要假设是一种源于黄体的化合物(S) 与黄体酮联合作用启动新的胚胎 在雪貂和一种表现出 刻意延迟植入。第一个目标是确定 卵巢因子的化学性质(脂或蛋白质)，不包括 黄体酮，它能诱导雪貂着床。这将是 通过从获得的卵巢中提取蛋白质和脂肪来完成的 从怀孕第八天的雪貂身上检测出 以及分离的脂类和蛋白质诱导着床的能力 在去卵巢(第6天)用黄体酮处理的雪貂和正常 斑点臭鼬的胚泡植入延迟。在将军之后 卵巢植入因子的化学性质被确定，尝试 将进一步对该化合物进行表征或鉴定(S)。尝试 将验证黄体(CL)是 化合物，通过培养第8天从雪貂获得的CL标记 前体，然后使条件培养基受相同的 生产生物活性产品的分馏过程 能够诱导植入的。最后，我们将尝试 确定卵巢着床因子是否刺激胚胎 通过直接作用于胚泡或通过其 对子宫的作用。这将通过刷新 原位取自子宫的囊胚及囊胚变化的比较 直径、~(14)C-葡萄糖利用和蛋白质、RNA和DNA的合成 在短期文化存在或不存在的情况下 部分纯化的卵巢因子的浓度。卵巢 这些同样的动物(现在他们的子宫)也会受到同样的影响 缺乏胚泡)几天，子宫检查有无变化 3 H-尿苷和3 H-胸腺嘧啶核苷掺入RNA和DNA。《长河》 拟议研究的长期目标是定义 垂体前叶和卵巢的激素相互作用控制 胚胎发育的停止和随后的重新启动 表现出一定的着床延迟的哺乳动物。

The primary hypothesis to be tested is that a compound(s) of luteal origin acts in conjunction with progesterone to initiate renewed embryonic development and implantation in the ferret and in a species exhibiting an obligate delay of implantation. The first objective is to determine the chemical nature (lipid or protein) of the ovarian factor, other than progesterone, which induces implantation in ferrets. This will be accomplished by extracting the proteins and lipids from ovaries obtained from ferrets on the eighth day of pregnancy and testing the unfractionated and fractioned lipids and proteins for their ability to induce implantation in ovariectomized (Day 6) progesterone-treated ferrets and in intact spotted skunks with delayed implanting blastocysts. After the general chemical nature of the ovarian implantation factor is determined, attempts will be made to further characterize or identify the compound(s). Attempts will be made to verify that corpora lutea (CL) are a source of the compound, by culturing day eight CL obtained from ferrets with labeled precursors and then subjecting the conditioned culture medium to the same fractionation procedure which produced a biologically active product capable of inducing implantation. Lastly, an attempt will be made to determine whether the ovarian implantation factor stimulates embryonic development by acting directly on the blastocyst or indirectly through its action on the uterus. This will be accomplished by flushing the blastocysts from the uterus in situ and comparing changes in blastocyst diameter, 14C-glucose utilization and synthesis of protein, RNA, and DNA after short-term culture in the presence or absence of varying concentrations of the partially purified ovarian factor. The ovarian factor will likewise be administered to these same animals (whose uteri now lack blastocysts) for several days, and the uteri examined for changes in incorporation of 3H-uridine and 3H-thymidine into RNA and DNA. The long term goal of the proposed research is to define the mechanisms by which hormones of the anterior pituitary and ovary interact in controlling the cessation and the subsequent reinitiation of embryonic development in mammals exhibiting an obligate delay of implantation.